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SFTPD ELISA 试剂盒

This 人 SFTPD ELISA Kit is a Colorimetric ELISA Kit designed to quantify 人 SFTPD.
Rockland
产品编号 ABIN6720259
Supplier Product No.: koa0674
发货至: 中国

Quick Overview for SFTPD ELISA 试剂盒 (ABIN6720259)

抗原

See all SFTPD ELISA试剂盒
SFTPD (Surfactant Protein D (SFTPD))

适用

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检测方法

Colorimetric

实验类型

Sandwich ELISA

检测范围

625 pg/mL - 40000 pg/mL

应用范围

ELISA

样品类型

Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
  • 最低检测浓度

    625 pg/mL

    Supplier Product No.

    koa0674

    Supplier

    Rockland

    原理

    Human SP-D Sandwich ELISA Kit for Quantitative Detection

    品牌

    AccuSignal™

    Analytical Method

    Quantitative

    特异性

    Production: Natural and recombinant human SP-D. There is no detectable cross-reactivity with other relevant proteins.

    灵敏度

    < 20 pg/mL

    组件

    • Antibody-coated 96-well plate
    • Target Protein Standard
    • Detection antibody
    • Detection reagent
    • Diluent buffers
    • Wash buffers
    • Substrate Solution
    • Stop solutions
    • Adhesive covers
  • 应用备注

    Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 40,000pg/mL, 20,000pg/mL, 10,000pg/mL, 5,000pg/mL, 2,500pg/mL, 1,250pg/mL, 625pg/mL human SP-D standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human SP-D standard solution and each sample be measured in duplicate.

    说明

    Standard: Expression system for standard: NSO, Immunogen sequence: A21-F375

    样本量

    100 μL

    板类型

    Pre-coated

    限制

    仅限研究用
  • 储存条件

    4 °C,-20 °C

    储存方法

    Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

    有效期

    12 months
  • 抗原 See all SFTPD ELISA试剂盒

    SFTPD (Surfactant Protein D (SFTPD))

    别名

    SP-D

    背景

    Synonyms: COLEC 7, COLEC7, Collectin-7, Collectin7, Lung surfactant protein D, PSP D, PSP-D, PSP-D Surfactant protein D, PSPD, Pulmonary surfactant apoprotein, Pulmonary surfactant associated protein D, Pulmonary surfactant associated protein PSP-D, Pulmonary surfactant-associated protein D, SFTP 4, SFTP4, SFTPD, SFTPD_HUMAN, SP D, SP-D, Surfactant associated protein pulmonary 4, Surfactant pulmonary associated protein D

    Background: Surfactant, pulmonary-associated protein D, also known as SFTPD or SP-D, is a protein which in humans is encoded by the SFTPD gene. By fluorescence in situ hybridization, the SP-D gene was localized in 10q22.2-q23.1. On the basis of homology with other collectins, potential functions for SP-D include roles in innate immunity and surfactant metabolism, SP-D is produced in the bronchiolar and terminal epithelium of human fetal lung from about 21 weeks of gestation. What's more, SP-A and SP-D act as dual-function surveillance molecules that reverse orientation and function and become initiators of host-defense reactions.

    基因ID

    6441

    NCBI登录号

    NP_003010

    UniProt

    P35247
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