PLAUR ELISA 试剂盒
Quick Overview for PLAUR ELISA 试剂盒 (ABIN6720257)
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检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 62.5 pg/mL
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Supplier Product No.
- koa0708
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Supplier
- Rockland
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原理
- Mouse uPAR Sandwich ELISA Kit for Quantitative Detection
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品牌
- AccuSignal™
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Analytical Method
- Quantitative
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特异性
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Production: Natural and recombinant mouse uPAR. There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- < 5 pg/mL
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组件
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- Antibody-coated 96-well plate
- Target Protein Standard
- Detection antibody
- Detection reagent
- Diluent buffers
- Wash buffers
- Substrate Solution
- Stop solutions
- Adhesive covers
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Plasminogen Activator, Urokinase Receptor (PLAUR) ELISA Kit
PLAUR 适用: 人 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Plasminogen Activator, Urokinase Receptor (PLAUR) ELISA KitPLAUR 适用: 小鼠 Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
Plasminogen Activator, Urokinase Receptor (PLAUR) ELISA KitPLAUR 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Plasminogen Activator, Urokinase Receptor (PLAUR) ELISA KitPLAUR 适用: 大鼠 Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
Plasminogen Activator, Urokinase Receptor (PLAUR) ELISA KitPLAUR 适用: 人 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Cell Culture Supernatant, Plasma, Serum
Plasminogen Activator, Urokinase Receptor (PLAUR) ELISA KitPLAUR 适用: 大鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Plasma, Serum
Plasminogen Activator, Urokinase Receptor (PLAUR) ELISA KitPLAUR 适用: 小鼠 AA 24-297 Colorimetric Sandwich ELISA 62.5-4000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Plasminogen Activator, Urokinase Receptor (PLAUR) ELISA KitPLAUR 适用: 人 AA 23-303 Colorimetric Sandwich ELISA 62.5-4000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum, Urine
Plasminogen Activator, Urokinase Receptor (PLAUR) ELISA KitPLAUR 适用: 人 Colorimetric Sandwich ELISA 32-2000 pg/mL Cell Lysate, Plasma, Serum
Plasminogen Activator, Urokinase Receptor (PLAUR) ELISA KitPLAUR 适用: 人 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
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应用备注
- Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL mouse uPAR standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each mouse uPAR standard solution and each sample be measured in duplicate.
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说明
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Standard: Expression system for standard: NSO, Immunogen sequence: L24-T297
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样本量
- 100 μL
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板类型
- Pre-coated
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限制
- 仅限研究用
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
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有效期
- 12 months
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- PLAUR (Plasminogen Activator, Urokinase Receptor (PLAUR))
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别名
- uPAR
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背景
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Synonyms: CD87, Monocyte activation antigen Mo3, PLAUR, U-PAR, uPAR, UPAR_HUMAN, Urokinase plasminogen activator surface receptor
Background: The urokinase-type plasminogen activator receptor(uPAR) is a key molecule in the regulation of cell-surface plasminogen activation and, as such, plays an important role in many normal as well as pathological processes.1 The cDNA for Mo3, an activation antigen expressed by human monocytes and myelomonocytic cell lines after stimulation by a variety of agents. Mo3 expression in vivo is associated predominantly with macrophages in inflammatory sites. It is a highly glycosylated protein of about 50 kD in monocytes where it is anchored to the plasma membrane by glycosyl-phosphatidylinositol linkage. The complete coding sequence of the cDNA has been found to encode 419 amino acids including a predicted signal peptide of 22 residues and a hydrophobic C-terminal portion. Mo3 is identical to the human receptor for the urokinase plasminogen activator.2 UPAR is a useful prognostic marker for biologically aggressive forms of endometrial cancer.3 PLAUR is located at chromosome 19q13.1-q13.2.1
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基因ID
- 18793
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NCBI登录号
- NP_035243
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UniProt
- P35456
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途径
- Inositol Metabolic Process
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