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TDGF1 ELISA 试剂盒

This 小鼠 TDGF1 ELISA Kit is a Colorimetric ELISA Kit designed to quantify 小鼠 TDGF1.
Rockland
产品编号 ABIN6720248
Supplier Product No.: koa0848
发货至: 中国

Quick Overview for TDGF1 ELISA 试剂盒 (ABIN6720248)

抗原

See all TDGF1 ELISA试剂盒
TDGF1 (Teratocarcinoma-Derived Growth Factor 1 (TDGF1))

适用

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小鼠

检测方法

Colorimetric

实验类型

Sandwich ELISA

检测范围

62.5 pg/mL - 4000 pg/mL

应用范围

ELISA

样品类型

Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
  • 最低检测浓度

    62.5 pg/mL

    Supplier Product No.

    koa0848

    Supplier

    Rockland

    原理

    Mouse Cripto - TDGF1 Sandwich ELISA Kit for Quantitative Detection

    品牌

    AccuSignal™

    Analytical Method

    Quantitative

    特异性

    Production: Natural and recombinant mouse Cripto. There is no detectable cross-reactivity with other relevant proteins.

    灵敏度

    < 10 pg/mL

    组件

    • Antibody-coated 96-well plate
    • Target Protein Standard
    • Detection antibody
    • Detection reagent
    • Diluent buffers
    • Wash buffers
    • Substrate Solution
    • Stop solutions
    • Adhesive covers
  • 应用备注

    Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL mouse Cripto standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. We recommend that each mouse Cripto standard solution and each sample is measured in duplicate.

    说明

    Standard: Expression system for standard: NSO, Immunogen sequence: R26-Q150

    样本量

    100 μL

    板类型

    Pre-coated

    限制

    仅限研究用
  • 储存条件

    4 °C,-20 °C

    储存方法

    Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

    有效期

    12 months
  • 抗原 See all TDGF1 ELISA试剂盒

    TDGF1 (Teratocarcinoma-Derived Growth Factor 1 (TDGF1))

    别名

    Cripto/TDGF1

    背景

    Synonyms: CR, CRGF, Cripto 1, Cripto 1 growth factor, cripto, Cripto-1 growth factor, Cripto1, Cripto1 growth factor, Epidermal growth factor like cripto protein CR1, Epidermal growth factor-like cripto protein CR1, TDGF 1, TDGF1, TDGF1_HUMAN, Teratocarcinoma derived growth factor 1, Teratocarcinoma-derived growth factor 1

    Background: Teratocarcinoma-derived growth factor 1 is a protein that in humans is encoded by the TDGF1 gene. It is mapped to 3p23-p21. The protein is an extracellular, membrane-bound signaling protein that plays an essential role in embryonic development and tumor growth. Mutations in this gene are associated with forebrain defects. Pseudogenes of this gene are found on chromosomes 2, 3, 6, 8, 19 and X. Alternate splicing results in multiple transcript variants.

    基因ID

    21667

    NCBI登录号

    NP_033545

    UniProt

    P51865

    途径

    EGFR Signaling Pathway
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