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CD48 ELISA 试剂盒

This 小鼠 CD48 ELISA Kit is a Colorimetric ELISA Kit designed to quantify 小鼠 CD48.
Rockland
产品编号 ABIN6720244
Supplier Product No.: koa0787
发货至: 中国

Quick Overview for CD48 ELISA 试剂盒 (ABIN6720244)

抗原

See all CD48 ELISA试剂盒
CD48

适用

  • 4
  • 3
  • 1
小鼠

检测方法

Colorimetric

实验类型

Sandwich ELISA

检测范围

62.5 pg/mL - 4000 pg/mL

应用范围

ELISA

样品类型

Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
  • 最低检测浓度

    62.5 pg/mL

    Supplier Product No.

    koa0787

    Supplier

    Rockland

    原理

    Mouse CD48 Sandwich ELISA Kit for Quantitative Detection

    品牌

    AccuSignal™

    Analytical Method

    Quantitative

    特异性

    Production: Natural and recombinant mouse CD48. There is no detectable cross-reactivity with other relevant proteins.

    灵敏度

    < 10 pg/mL

    组件

    • Antibody-coated 96-well plate
    • Target Protein Standard
    • Detection antibody
    • Detection reagent
    • Diluent buffers
    • Wash buffers
    • Substrate Solution
    • Stop solutions
    • Adhesive covers
  • 应用备注

    Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL mouse CD48 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each mouse CD48 standard solution and each sample be measured in duplicate.

    说明

    Standard: Expression system for standard: NSO, Immunogen sequence: F23-S217

    样本量

    100 μL

    板类型

    Pre-coated

    限制

    仅限研究用
  • 储存条件

    4 °C,-20 °C

    储存方法

    Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

    有效期

    12 months
  • 抗原 See all CD48 ELISA试剂盒

    CD48

    别名

    CD48

    背景

    Synonyms: Antigen CD48, B cell membrane protein, B lymphocyte activation marker BLAST 1, B lymphocyte activation marker BLAST 1, B-cell activation marker, B-lymphocyte activation marker BLAST-1, BCM 1 surface antigen, BCM1, BCM1 surface antigen, BLAST, BLAST1, CD48 antigen (B cell membrane protein), CD48 antigen, CD48 Molecule, CD48 protein, CD48_HUMAN, hCD48, Leucocyte antigen MEM 102 mCD48, MEM102, Signaling lymphocytic activation molecule 2, SLAM family member 2, SLAMF 2, SLAMF2

    Background: CD48 antigen(Cluster of Differentiation 48), also known as BLAST-1 or SLAMF2, is a protein that in humans is encoded by the CD48 gene. CD48 is a member of the CD2 subfamily of the immunoglobulin superfamily(IgSF) which includes SLAM(signaling lymphocyte activation molecules) proteins, such as CD84, CD150, CD229 and CD244. It is mapped to 1q23.3. CD48 is found on the surface of lymphocytes and other immune cells, dendritic cells and endothelial cells, and participates in activation and differentiation pathways in these cells. The encoded protein does not have a transmembrane domain, however, but is held at the cell surface by a GPI anchor via a C-terminal domain which maybe cleaved to yield a soluble form of the receptor.

    基因ID

    12506

    NCBI登录号

    NP_031675

    UniProt

    P18181
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