LTA ELISA 试剂盒
Quick Overview for LTA ELISA 试剂盒 (ABIN6720236)
抗原
See all LTA ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 62.5 pg/mL
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Supplier Product No.
- koa0358
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Supplier
- Rockland
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原理
- Human TNF beta Sandwich ELISA Kit for Quantitative Detection
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品牌
- AccuSignal™
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Analytical Method
- Quantitative
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特异性
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Production: Natural and recombinant human TNF beta. There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- < 5 pg/mL
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组件
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- Antibody-coated 96-well plate
- Target Protein Standard
- Detection antibody
- Detection reagent
- Diluent buffers
- Wash buffers
- Substrate Solution
- Stop solutions
- Adhesive covers
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Lymphotoxin-alpha (LTA) ELISA Kit
LTA 适用: 大鼠 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Lymphotoxin-alpha (LTA) ELISA KitLTA 适用: Pig Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Lymphotoxin-alpha (LTA) ELISA KitLTA 适用: 犬 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Lymphotoxin-alpha (LTA) ELISA KitLTA 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Lymphotoxin-alpha (LTA) ELISA KitLTA 适用: 小鼠 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Lymphotoxin-alpha (LTA) ELISA KitLTA 适用: 兔 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Lymphotoxin-alpha (LTA) ELISA KitLTA 适用: Cow Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Lymphotoxin-alpha (LTA) ELISA KitSmall Sample LTA 适用: 小鼠 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Lymphotoxin-alpha (LTA) ELISA KitLTA 适用: 化学剂 AA 35-205 Colorimetric Sandwich ELISA 62.5-4000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum
Lymphotoxin-alpha (LTA) ELISA KitLTA 适用: 化学剂 Colorimetric Sandwich ELISA 60-18000 pg/mL Cell Culture Supernatant, Plasma, Serum
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应用备注
- Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL human TNF beta standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA, citrate) to each empty well. It is recommended that each human TNF beta standard solution and each sample be measured in duplicate.
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说明
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Standard: Expression system for standard: E.coli, Immunogen sequence: L35-L205
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样本量
- 100 μL
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板类型
- Pre-coated
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限制
- 仅限研究用
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
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有效期
- 12 months
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- LTA (Lymphotoxin-alpha (LTA))
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别名
- TNF beta
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物质类
- Chemical
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背景
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Synonyms: Coley's toxin, DAMA-25N12.13-004, DIF, Differentiation inducing factor, Hemorrhagic factor, LT, LT alpha, LT-alpha, Lta, LTalpha, Lymphotoxin alpha, Lymphotoxin alpha (TNF superfamily, member 1), Lymphotoxin-alpha, Natural killer cytotoxic factor, Necrosin, NKCF, TNF B, TNF superfamily member 1, TNF-beta, TNFB, TNFB_HUMAN, TNFbeta, TNFSF 1, TNFSF1, Tumor necrosis factor beta, Tumor necrosis factor ligand superfamily member 1, Tumor necrosis factor superfamily member 1
Background: Tumor necrosis factor-beta(TNF-beta), previously called lymphotoxin-alpha(LTA), is a cytokine produced by lymphocytes. TNF-alpha and TNF-beta share 35 % identity and 50 % homology in the amino acid sequence. The substance LTA mediates a wide variety of inflammatory, immunostimulatory, and antiviral responses. LTA is associated with susceptibility to myocardial infarction, asthma and other diseases. The LTA gene is located on human chromosome 6. The standard product used in this kit is recombinant protein, consisting of 172 amino acids with the molecular mass of 18.8KDa.
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基因ID
- 4049
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NCBI登录号
- NP_000586
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UniProt
- P01374
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途径
- Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process
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