F11R ELISA 试剂盒
Our Local Distributor
北京 101111
Quick Overview for F11R ELISA 试剂盒 (ABIN6720201)
抗原
See all F11R ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
-
-
最低检测浓度
- 31.2 pg/mL
-
Supplier Product No.
- koa0721
-
Supplier
- Rockland
-
原理
- Mouse JAM A - F11R Sandwich ELISA Kit for Quantitative Detection
-
品牌
- AccuSignal™
-
Analytical Method
- Quantitative
-
特异性
-
Expression system for standard: NS0
Immunogen sequence: K27-G238 -
交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
-
灵敏度
- < 10 pg/mL
-
组件
-
- Antibody-coated 96-well plate
- Target Protein Standard
- Detection antibody
- Detection reagent
- Diluent buffers
- Wash buffers
- Substrate Solution
- Stop solutions
- Adhesive covers
-
试剂未包括
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.
-
-
F11 Receptor (F11R) ELISA Kit
F11R 适用: 人 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
F11 Receptor (F11R) ELISA KitF11R 适用: Cow Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
F11 Receptor (F11R) ELISA KitF11R 适用: 人 AA 28-238 Colorimetric Sandwich ELISA 46.9 pg/mL - 3000 pg/mL Cell Culture Supernatant, Serum
F11 Receptor (F11R) ELISA KitF11R 适用: 小鼠 AA 27-238 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma (EDTA), Plasma (heparin), Serum
F11 Receptor (F11R) ELISA KitF11R 适用: 小鼠 Colorimetric Sandwich ELISA 8 pg/mL - 2000 pg/mL Cell Culture Supernatant, Plasma, Serum
F11 Receptor (F11R) ELISA KitF11R 适用: Cow Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
-
-
说明
-
Capture antibody and Detection antibody: monoclonal antibody from rat, polyclonal antibody from goat
-
样本量
- 100 μL
-
板类型
- Pre-coated
-
实验流程
- Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL mouse JAM-A standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each mouse JAM-A standard solution and each sample be measured in duplicate.
-
实验精密度
-
Intra-Assay Precision (CV%): 4.3%, 7.8%, 6.6%
Inter-Assay Precision (CV%): 4.9%, 8.3%, 7.8% -
限制
- 仅限研究用
-
-
-
储存条件
- 4 °C,-20 °C
-
储存方法
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
-
有效期
- 12 months
-
-
- F11R (F11 Receptor (F11R))
-
别名
- JAM-A/F11R
-
背景
-
Background: Junctional adhesion molecule A (JAM-A) is a protein that in humans is encoded by the F11R gene. It is mapped to 1q23.3. This gene is an immunoglobulin-like molecule that colocalizes with tight junctions in endothelium and epithelium and is also found on blood leukocytes and platelets. JAM-A plays an important role in the regulation of tight junction assembly in epithelia. In addition, it can act as a receptor for reovirus, a ligand for the integrin LFA1, involved in leukocyte transmigration, and a platelet receptor. JAM-A has a nonredundant role in controlling DC motility, trafficking to lymph nodes, and activation of specific immunity.
Gene Full Name: F11 receptor
-
基因ID
- 16456
-
NCBI登录号
- NP_766235
-
UniProt
- O88792
-
途径
- Cell-Cell Junction Organization
抗原 See all F11R ELISA试剂盒
-
