ELANE ELISA 试剂盒
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Quick Overview for ELANE ELISA 试剂盒 (ABIN6720191)
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检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 31.2 pg/mL
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Supplier Product No.
- koa0872
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Supplier
- Rockland
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原理
- Mouse Elastase - ELANE - ELA2 Sandwich ELISA Kit for Quantitative Detection
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品牌
- AccuSignal™
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Analytical Method
- Quantitative
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特异性
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Expression system for standard: NS0
Immunogen sequence: S27-R260 -
交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- < 10 pg/mL
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组件
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- Antibody-coated 96-well plate
- Target Protein Standard
- Detection antibody
- Detection reagent
- Diluent buffers
- Wash buffers
- Substrate Solution
- Stop solutions
- Adhesive covers
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试剂未包括
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.
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Elastase, Neutrophil Expressed (ELANE) ELISA Kit
ELANE 适用: 小鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum
Elastase, Neutrophil Expressed (ELANE) ELISA KitELANE 适用: 大鼠 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Plasma, Serum
Elastase, Neutrophil Expressed (ELANE) ELISA KitELANE 适用: 人 Colorimetric Sandwich ELISA 0.19 ng/mL - 12 ng/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Elastase, Neutrophil Expressed (ELANE) ELISA KitELANE 适用: 犬 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Plasma, Serum
Elastase, Neutrophil Expressed (ELANE) ELISA KitELANE 适用: 大鼠 Colorimetric Sandwich ELISA 12.5 ng/mL - 800 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Elastase, Neutrophil Expressed (ELANE) ELISA KitELANE 适用: 人 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Elastase, Neutrophil Expressed (ELANE) ELISA KitELANE 适用: 小鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Elastase, Neutrophil Expressed (ELANE) ELISA KitELANE 适用: Pig Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Elastase, Neutrophil Expressed (ELANE) ELISA KitELANE 适用: 大鼠 Colorimetric Sandwich ELISA 1.563 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
Elastase, Neutrophil Expressed (ELANE) ELISA KitELANE 适用: 人 Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Cell Culture Supernatant, Plasma, Serum
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说明
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Capture antibody and Detection antibody: a monoclonal antibody from rat, a biotinylated polyclonal antibody from goat
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样本量
- 100 μL
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板类型
- Pre-coated
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实验流程
- Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL mouse Elastase standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each mouse Elastase standard solution and each sample be measured in duplicate.
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实验精密度
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Intra-Assay Precision (CV%): 7.9%, 5.3%, 7.7%
Inter-Assay Precision (CV%): 8.1%, 6%, 7.8% -
限制
- 仅限研究用
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
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有效期
- 12 months
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- ELANE (Elastase, Neutrophil Expressed (ELANE))
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别名
- Elastase/ELANE/ELA2
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背景
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Background: Neutrophil elastase is a serine protease of neutrophil and monocyte granules. Its key physiologic role is in innate host defense, but it can also participate in tissue remodeling and possesses secretagogue actions important to local inflammatory responses. Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode structurally similar proteins. The encoded preproprotein is proteolytically processed to generate the active protease. Following activation, this protease hydrolyzes proteins within specialized neutrophil lysosomes, called azurophil granules, as well as proteins of the extracellular matrix. This protein also degrades the outer membrane protein A (OmpA) of E. coli as well as the virulence factors of such bacteria as Shigella, Salmonella and Yersinia. Mutations in this gene are associated with cyclic neutropenia and severe congenital neutropenia (SCN). This gene is present in a gene cluster on chromosome 19.
Gene Full Name: elastase, neutrophil expressed
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基因ID
- 50701
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NCBI登录号
- NP_056594
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UniProt
- Q3UP87
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