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Decorin ELISA 试剂盒

This 小鼠 Decorin ELISA Kit is a Colorimetric ELISA Kit designed to quantify 小鼠 Decorin.
Rockland
产品编号 ABIN6720190
Supplier Product No.: koa0442
发货至: 中国

Quick Overview for Decorin ELISA 试剂盒 (ABIN6720190)

抗原

See all Decorin (DCN) ELISA试剂盒
Decorin (DCN)

适用

  • 10
  • 5
  • 5
  • 5
  • 4
  • 3
  • 2
  • 2
  • 2
  • 1
  • 1
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  • 1
  • 1
小鼠

检测方法

Colorimetric

实验类型

Sandwich ELISA

检测范围

31.2 pg/mL - 2000 pg/mL

应用范围

ELISA

样品类型

Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
  • 最低检测浓度

    31.2 pg/mL

    Supplier Product No.

    koa0442

    Supplier

    Rockland

    原理

    Mouse Decorin Sandwich ELISA Kit for Quantitative Detection

    品牌

    AccuSignal™

    Analytical Method

    Quantitative

    特异性

    Production: Natural and recombinant human Decorin. There is no detectable cross-reactivity with other relevant proteins.

    灵敏度

    < 10 pg/mL

    组件

    • Antibody-coated 96-well plate
    • Target Protein Standard
    • Detection antibody
    • Detection reagent
    • Diluent buffers
    • Wash buffers
    • Substrate Solution
    • Stop solutions
    • Adhesive covers
  • 应用备注

    Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human Decorin standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human Decorin standard solution and each sample be measured in duplicate.

    说明

    Standard: Expression system for standard: NSO, Immunogen sequence: D31-K354

    样本量

    100 μL

    板类型

    Pre-coated

    限制

    仅限研究用
  • 储存条件

    4 °C,-20 °C

    储存方法

    Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

    有效期

    12 months
  • 抗原 See all Decorin (DCN) ELISA试剂盒

    Decorin (DCN)

    别名

    Decorin

    背景

    Synonyms: Bone proteoglycan II, CSCD, DCN, DCN protein, Decorin, Decorin proteoglycan, Dermatan sulphate proteoglycans II, DKFZp686J19238, DSPG 2, DSPG2, PG 40 , PG II, PG S2, PG-S2, PG40, PGII, PGS 2, PGS2, PGS2_HUMAN, Proteoglycan core protein, SLRR1B, Small leucine rich protein 1B

    Background: Decorin is a proteoglycan on average 90 - 140 kilodaltons(kD) in size. It belongs to the small leucine-rich proteoglycan(SLRP) family and consists of a protein core containing leucine repeats with a glycosaminoglycan(GAG) chain consisting of either chondroitin sulfate(CS) or dermatan sulfate(DS).Using a genomic clone as the labeled probe and in situ hybridization of human metaphase chromosomes, decorin gene was mapped to the discrete region of human chromosome 12q23. Decorin is capable of suppressing the growth of various tumor cell lines when expressed ectopically.

    基因ID

    13179

    NCBI登录号

    NP_001177380

    UniProt

    P28654

    途径

    Glycosaminoglycan Metabolic Process
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