ESM1 ELISA 试剂盒
Quick Overview for ESM1 ELISA 试剂盒 (ABIN6720165)
抗原
See all ESM1 ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 31.2 pg/mL
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Supplier Product No.
- koa0444
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Supplier
- Rockland
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原理
- Human ESM1 - Endocan Sandwich ELISA Kit for Quantitative Detection
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品牌
- AccuSignal™
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Analytical Method
- Quantitative
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特异性
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Production: Natural and recombinant human ESM1. There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- < 10 pg/mL
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组件
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- Antibody-coated 96-well plate
- Target Protein Standard
- Detection antibody
- Detection reagent
- Diluent buffers
- Wash buffers
- Substrate Solution
- Stop solutions
- Adhesive covers
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Endothelial Cell-Specific Molecule 1 (ESM1) ELISA Kit
ESM1 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Endothelial Cell-Specific Molecule 1 (ESM1) ELISA KitESM1 适用: 小鼠 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Endothelial Cell-Specific Molecule 1 (ESM1) ELISA KitESM1 适用: 大鼠 Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
Endothelial Cell-Specific Molecule 1 (ESM1) ELISA KitESM1 适用: 人 AA 20-184 Colorimetric Sandwich ELISA 31.2-2000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Endothelial Cell-Specific Molecule 1 (ESM1) ELISA KitESM1 适用: 小鼠 AA 20-184 Colorimetric Sandwich ELISA 23.4-1500 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Endothelial Cell-Specific Molecule 1 (ESM1) ELISA KitESM1 适用: 小鼠 Colorimetric Sandwich ELISA 0.1-30 ng/mL Cell Culture Supernatant, Plasma, Serum
Endothelial Cell-Specific Molecule 1 (ESM1) ELISA KitESM1 适用: 小鼠 Colorimetric Sandwich ELISA 23.4 pg/mL - 1500 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
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应用备注
- Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human ESM1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human ESM1 standard solution and each sample be measured in duplicate.
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说明
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Standard: Expression system for standard: NSO, Immunogen sequence: W20-R184
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样本量
- 100 μL
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板类型
- Pre-coated
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限制
- 仅限研究用
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
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有效期
- 12 months
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- ESM1 (Endothelial Cell-Specific Molecule 1 (ESM1))
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别名
- ESM1/Endocan
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背景
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Synonyms: Endothelial cell-specific molecule 1, ESM-1, Esm1, ESM1_HUMAN
Background: Endothelial cell-specific molecule 1, also known as Endocan, is a protein that in humans is encoded by the ESM1 gene. This gene encodes a secreted protein which is mainly expressed in the endothelial cells in human lung and kidney tissues. The expression of this gene is regulated by cytokines, suggesting that it may play a role in endothelium-dependent pathological disorders. The transcript contains multiple polyadenylation and mRNA instability signals. ESM1 has been described as a specific biomarker of tip cells during neoangiogenesis by independent teams. Its expression has been shown to be increase in presence of pro-angiogenic growth factors such as VEGF(vascular endothelial growth factor) or FGF-2 (fibroblast growth factor 2).
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基因ID
- 11082
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NCBI登录号
- NP_001129076
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UniProt
- Q9NQ30
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途径
- Growth Factor Binding
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