Endothelin ELISA 试剂盒
Quick Overview for Endothelin ELISA 试剂盒 (ABIN6720146)
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See all Endothelin ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 3.9 pg/mL
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Supplier Product No.
- koa0582
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Supplier
- Rockland
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原理
- Rat Endothelin Sandwich ELISA Kit for Quantitative Detection
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品牌
- AccuSignal™
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Analytical Method
- Quantitative
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特异性
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Production: Natural and recombinant rat Endothelin. There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- < 0.5 pg/mL
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组件
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- Antibody-coated 96-well plate
- Target Protein Standard
- Detection antibody
- Detection reagent
- Diluent buffers
- Wash buffers
- Substrate Solution
- Stop solutions
- Adhesive covers
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Endothelin ELISA Kit
适用: 小鼠 Colorimetric Sandwich ELISA 3.9 pg/mL - 250 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
Endothelin ELISA Kit适用: 人 Colorimetric Sandwich ELISA 3.9 pg/mL - 250 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
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应用备注
- Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 16.5pg/mL, 7.8pg/mL, 3.9pg/mL rat Endothelin standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of rat cell culture supernatants, serum or plasma(heparin, EDTA) to each empty well. We recommend that each rat Endothelin standard solution and each sample is measured in duplicate.
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说明
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Standard: Expression system for standard: , Immunogen sequence: C53-W73
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样本量
- 100 μL
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板类型
- Pre-coated
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限制
- 仅限研究用
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
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有效期
- 12 months
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- Endothelin
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背景
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Synonyms: Big endothelin 1, ET1, HDLCQ7, PPET1, Preproendothelin 1, EDN2, EDN2_HUMAN, Endothelin 2, Endothelin-2, ET 2, ET-2, ET2, PPET2, Preproendothelin 2, Preproendothelin-2, EDN3, EDN3_HUMAN, Endothelin-3, ET 3, ET-3, PPET3, Preproendothelin 3, Preproendothelin-3
Background: The endothelins is a family of structurally and pharmacologically distinct peptides, which has been identified and sequenced in humans. Three isoforms of human endothelin have been identified: endothelin-1, -2, and -3. Endothelin-1 is a potent, 21-amino acid vasoconstrictor peptide produced by vascular endothelial cells. Endothelins are 21-amino acid vasoconstricting peptides produced primarily in the endothelium having a key role in vascular homeostasis. Endothelins are implicated in vascular diseases of several organ systems, including the heart, general circulation and brain. Endothelins are proteins that constrict blood vessels and raise blood pressure. They are normally kept in balance by other mechanisms, but when they are over-expressed, they contribute to high blood pressure(hypertension) and heart disease.
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基因ID
- 24323
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NCBI登录号
- NP_036680
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UniProt
- P22388
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