VCAM1 ELISA 试剂盒
Quick Overview for VCAM1 ELISA 试剂盒 (ABIN6720137)
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See all VCAM1 ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 156 pg/mL
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Supplier Product No.
- koa0328
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Supplier
- Rockland
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原理
- Mouse VCAM-1 Sandwich ELISA Kit for Quantitative Detection
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品牌
- AccuSignal™
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Analytical Method
- Quantitative
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特异性
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Production: Natural and recombinant mouse VCAM-1. There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- < 5 pg/mL
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组件
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- Antibody-coated 96-well plate
- Target Protein Standard
- Detection antibody
- Detection reagent
- Diluent buffers
- Wash buffers
- Substrate Solution
- Stop solutions
- Adhesive covers
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Vascular Cell Adhesion Molecule 1 (VCAM1) ELISA Kit
VCAM1 适用: 人 Colorimetric Sandwich ELISA 1.563-100 ng/mL Plasma, Serum, Tissue Homogenate
Vascular Cell Adhesion Molecule 1 (VCAM1) ELISA KitVCAM1 适用: 小鼠 Colorimetric Sandwich ELISA 0.39 ng/mL - 25 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Vascular Cell Adhesion Molecule 1 (VCAM1) ELISA KitVCAM1 适用: 小鼠 Colorimetric Sandwich ELISA 39.062-2500 pg/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
Vascular Cell Adhesion Molecule 1 (VCAM1) ELISA KitVCAM1 适用: 大鼠 Colorimetric Sandwich ELISA 1.25 ng/mL - 80 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Vascular Cell Adhesion Molecule 1 (VCAM1) ELISA KitVCAM1 适用: 人 Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Vascular Cell Adhesion Molecule 1 (VCAM1) ELISA KitVCAM1 适用: 兔 Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Vascular Cell Adhesion Molecule 1 (VCAM1) ELISA KitVCAM1 适用: Pig Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
Vascular Cell Adhesion Molecule 1 (VCAM1) ELISA KitVCAM1 适用: 人 AA 25-698 Colorimetric Sandwich ELISA 156-10000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Vascular Cell Adhesion Molecule 1 (VCAM1) ELISA KitVCAM1 适用: 犬 Colorimetric Sandwich ELISA 3.12 ng/mL - 200 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Vascular Cell Adhesion Molecule 1 (VCAM1) ELISA KitVCAM1 适用: 猴 Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
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应用备注
- Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 313pg/mL, 156pg/mL mouse VCAM-1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. We recommend that each mouse VCAM-1 standard solution and each sample is measured in duplicate.
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说明
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Standard: Expression system for standard: NSO, Immunogen sequence: F25 - E698
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样本量
- 100 μL
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板类型
- Pre-coated
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限制
- 仅限研究用
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
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有效期
- 12 months
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- VCAM1 (Vascular Cell Adhesion Molecule 1 (VCAM1))
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别名
- VCAM-1
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背景
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Synonyms: CD106, CD106 antigen, DKFZp779G2333, INCAM 100, INCAM-100, INCAM100, L1CAM, MGC99561, V CAM 1, V-CAM 1, Vascular cell adhesion molecule 1, Vascular cell adhesion protein 1, VCAM 1, VCAM-1, VCAM1, VCAM1_HUMAN
Background: Vascular cell adhesion molecule 1(VCAM-1) is a cell surface glycoprotein adhesive for certain blood leukocytes and tumor cells, which is expressed by activated endothelium in a variety of pathologic conditions including atherosclerosis. Increased expression of VCAM1 is associated with a variety of chronic inflammatory conditions, making its expression and function a target for therapeutic intervention. Integrin alpha4beta1(VLA-4) and VCAM-1 facilitate a critical cell-cell adhesion event required for survival of endothelial and mural cells during vascularization. The VCAM1 gene is assigned to the 1p31-32 region of chromosome 1 based on the analysis of human-mouse hybrid cell lines and in situ hybridization. The standard product used in this kit is recombinant mouse VCAM-1, a dimeric protein linking with a disulfide bond. It consists of Phe25-Glu698 amino acid sequence of a single chain with the molecular mass of 101.9KDa. As a result of glycosylation, the molecular mass of 120KDa is revealed by SDS-PAGE.
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基因ID
- 22329
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NCBI登录号
- NP_035823
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UniProt
- P29533
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途径
- Carbohydrate Homeostasis
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