TPSAB1 ELISA 试剂盒
Quick Overview for TPSAB1 ELISA 试剂盒 (ABIN6720119)
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检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 156 pg/mL
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Supplier Product No.
- koa0539
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Supplier
- Rockland
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原理
- Human Tryptase - TPSAB1 B2 Sandwich ELISA Kit for Quantitative Detection
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品牌
- AccuSignal™
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Analytical Method
- Quantitative
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特异性
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Production: Natural and recombinant human Tryptase. There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- < 15 pg/mL
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组件
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- Antibody-coated 96-well plate
- Target Protein Standard
- Detection antibody
- Detection reagent
- Diluent buffers
- Wash buffers
- Substrate Solution
- Stop solutions
- Adhesive covers
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Tryptase alpha/beta 1 (TPSAB1) ELISA Kit
TPSAB1 适用: 人 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Plasma, Serum
Tryptase alpha/beta 1 (TPSAB1) ELISA KitTPSAB1 适用: 小鼠 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Plasma, Serum, Tissue Homogenate
Tryptase alpha/beta 1 (TPSAB1) ELISA KitTPSAB1 适用: 大鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Tryptase alpha/beta 1 (TPSAB1) ELISA KitTPSAB1 适用: 人 Colorimetric Sandwich ELISA 1.563 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
Tryptase alpha/beta 1 (TPSAB1) ELISA KitWide Range TPSAB1 适用: 人 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Plasma, Serum
Tryptase alpha/beta 1 (TPSAB1) ELISA KitTPSAB1 适用: 大鼠 Colorimetric Sandwich ELISA 0.625 ng/mL - 40 ng/mL Plasma, Serum, Urine
Tryptase alpha/beta 1 (TPSAB1) ELISA KitTPSAB1 适用: 犬 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
Tryptase alpha/beta 1 (TPSAB1) ELISA KitTPSAB1 适用: Pig Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Plasma, Serum
Tryptase alpha/beta 1 (TPSAB1) ELISA KitTPSAB1 适用: 马 Colorimetric Sandwich ELISA 0.62 ng/mL - 40 ng/mL Plasma, Serum
Tryptase alpha/beta 1 (TPSAB1) ELISA KitHigh Sensitivity TPSAB1 适用: 犬 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
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应用备注
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ELISA_Dilution: 156pg/mL-10000pg/mL
Application_Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human Tryptase standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human Tryptase standard solution and each sample be measured in duplicate.
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说明
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Standard: Expression system for standard: NSO, Immunogen sequence: M1-V275
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样本量
- 100 μL
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板类型
- Pre-coated
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限制
- 仅限研究用
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
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有效期
- 12 months
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- TPSAB1 (Tryptase alpha/beta 1 (TPSAB1))
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别名
- Tryptase/TPSAB1,B2
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背景
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Synonyms: Tryptase-1, Tryptase1, Tryptase 1, TPS1, TPS2, TPSB1, Tryptase-2, Tryptase2, Tryptase 2, Tryptase-II, TryptaseII, Tryptase II, TPSB2, Tryptase alpha/beta-1, Tryptase beta-2
Background: Tryptases are serine proteases implicated in asthma and are highly expressed in human mast cells. They are derived from at least 4 nonallelic genes clustered on chromosome 16p13.3: TPSAB1, which represents the alpha and beta-I tryptase alleles, TPSB2, which represents the beta-II and beta-III tryptase alleles, TPSG1, and TPSD1. Elevated levels of serum tryptase occur in both anaphylactic and anaphylactoid reactions, but a negative test does not exclude anaphylaxis. Tryptase is less likely to be elevated in food allergy reactions as opposed to other causes of anaphylaxis.
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基因ID
- 7177
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NCBI登录号
- NP_003285
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UniProt
- Q15661
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