PF4 ELISA 试剂盒
Quick Overview for PF4 ELISA 试剂盒 (ABIN6720101)
抗原
See all PF4 ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 156 pg/mL
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Supplier Product No.
- koa0423
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Supplier
- Rockland
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原理
- Human CXCL4 - PF4 Sandwich ELISA Kit for Quantitative Detection
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品牌
- AccuSignal™
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Analytical Method
- Quantitative
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特异性
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Production: Natural and recombinant human CXCL4. There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- < 15 pg/mL
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组件
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- Antibody-coated 96-well plate
- Target Protein Standard
- Detection antibody
- Detection reagent
- Diluent buffers
- Wash buffers
- Substrate Solution
- Stop solutions
- Adhesive covers
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Platelet Factor 4 (PF4) ELISA Kit
PF4 适用: 人 Colorimetric Sandwich ELISA 1.56 pg/mL - 100 pg/mL Cell Culture Supernatant, Platelet-Poor Plasma, Serum
Platelet Factor 4 (PF4) ELISA KitPF4 适用: 小鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Platelet-Poor Plasma, Serum, Tissue Homogenate
Platelet Factor 4 (PF4) ELISA KitPF4 适用: 大鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Platelet-Poor Plasma, Serum
Platelet Factor 4 (PF4) ELISA KitPF4 适用: 小鼠 Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
Platelet Factor 4 (PF4) ELISA KitPF4 适用: 人 Colorimetric Sandwich ELISA 1.563 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
Platelet Factor 4 (PF4) ELISA KitPF4 适用: 大鼠 Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Plasma, Serum, Tissue Homogenate
Platelet Factor 4 (PF4) ELISA KitPF4 适用: 兔 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Platelet-Poor Plasma, Serum
Platelet Factor 4 (PF4) ELISA KitPF4 适用: Cow Colorimetric Sandwich ELISA 6.25 pg/mL - 400 pg/mL Cell Culture Supernatant, Cell Lysate, Platelet-Poor Plasma, Serum, Tissue Homogenate
Platelet Factor 4 (PF4) ELISA KitPF4 适用: Pig Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Plasma
Platelet Factor 4 (PF4) ELISA KitPF4 适用: 大鼠 AA 30-105 Colorimetric Sandwich ELISA 156 pg/mL - 10000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
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应用备注
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ELISA_Dilution: 156pg/mL-10000pg/mL
Application_Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human CXCL4 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human CXCL4 standard solution and each sample be measured in duplicate.
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说明
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Standard: Expression system for standard: E.coli, Immunogen sequence: E32-S101
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样本量
- 100 μL
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板类型
- Pre-coated
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限制
- 仅限研究用
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
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有效期
- 12 months
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- PF4 (Platelet Factor 4 (PF4))
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别名
- CXCL4/PF4
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背景
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Synonyms: C X C motif chemokine 4, C-X-C motif chemokine 4, Chemokine (C X C motif) ligand 4, Chemokine (CXC motif) ligand 4, Chemokine, CXC motif, ligand 4, CXCL 4, CXCL4, Iroplact, MGC138298, Oncostatin A, Oncostatin-A, OncostatinA, PF 4, PF-4, PF4, Pf4a, Platelet factor 4, PLF4_HUMAN, RATPF4A, SCYB 4, SCYB4, short form, Small inducible cytokine subfamily B, member 4, Small inducible cytokine subfamily member 4
Background: Platelet factor 4 (PF4) is a small cytokine belonging to the CXC chemokine family that is also known as chemokine (C-X-C motif) ligand 4 (CXCL4). The PF4 gene was localized on 4q12-q13. Chemokines play fundamental roles in the development, homeostasis, and function of the immune system, and they have effects on cells of the central nervous system as well as on endothelial cells involved in angiogenesis or angiostasis. Platelet factor-4 is a 70-amino acid protein that is released from the alpha-granules of activated platelets and binds with high affinity to heparin. Its major physiologic role appears to be neutralization of heparin-like molecules on the endothelial surface of blood vessels, thereby inhibiting local antithrombin III activity and promoting coagulation. As a strong chemoattractant for neutrophils and fibroblasts, PF4 probably has a role in inflammation and wound repair.
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基因ID
- 5196
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NCBI登录号
- NP_002610
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UniProt
- P02776
抗原 See all PF4 ELISA试剂盒
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