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DMP1 ELISA 试剂盒

This 小鼠 DMP1 ELISA Kit is a Colorimetric ELISA Kit designed to quantify 小鼠 DMP1.
Rockland
产品编号 ABIN6720084
Supplier Product No.: koa0766
发货至: 中国

Quick Overview for DMP1 ELISA 试剂盒 (ABIN6720084)

抗原

See all DMP1 (DMTF1) ELISA试剂盒
DMP1 (DMTF1) (Cyclin D Binding Myb-Like Transcription Factor 1 (DMTF1))

适用

  • 3
  • 2
  • 1
  • 1
小鼠

检测方法

Colorimetric

实验类型

Sandwich ELISA

检测范围

156 pg/mL - 10000 pg/mL

应用范围

ELISA

样品类型

Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
  • 最低检测浓度

    156 pg/mL

    Supplier Product No.

    koa0766

    Supplier

    Rockland

    原理

    Mouse DMP-1 Sandwich ELISA Kit for Quantitative Detection

    品牌

    AccuSignal™

    Analytical Method

    Quantitative

    特异性

    Production: Natural and recombinant mouse DMP-1. There is no detectable cross-reactivity with other relevant proteins.

    灵敏度

    < 10 pg/mL

    组件

    • Antibody-coated 96-well plate
    • Target Protein Standard
    • Detection antibody
    • Detection reagent
    • Diluent buffers
    • Wash buffers
    • Substrate Solution
    • Stop solutions
    • Adhesive covers
  • 应用备注

    Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL mouse DMP-1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum, plasma(heparin, EDTA) or tissue homogenates to each empty well. It is recommended that each mouse DMP-1 standard solution and each sample be measured in duplicate.

    说明

    Standard: Expression system for standard: NSO, Immunogen sequence: L17-Y503

    样本量

    100 μL

    板类型

    Pre-coated

    限制

    仅限研究用
  • 储存条件

    4 °C,-20 °C

    储存方法

    Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

    有效期

    12 months
  • 抗原 See all DMP1 (DMTF1) ELISA试剂盒

    DMP1 (DMTF1) (Cyclin D Binding Myb-Like Transcription Factor 1 (DMTF1))

    别名

    DMP-1

    背景

    Synonyms: ARHP, ARHR, AV020965, Dentin matrix acidic phosphoprotein 1, Dentin matrix protein 1, DENTMAT, DMP 1, Dmp, MGC130441, PP, Serine rich acidic phosphoprotein

    Background: Dentin matrix acidic phosphorprotein 1 is a protein that in humans is encoded by the DMP1 gene. It belongs to the small integrin-binding ligand N-linked glycoprotein(SIBLING) family of secreted phosphoproteins. This gene is mapped to 4q22.1. This protein, which is critical for proper mineralization of bone and dentin, is present in diverse cells of bone and tooth tissues. In undifferentiated osteoblasts, DMP-1 is primarily a nuclear protein that regulates the expression of osteoblast-specific genes. During osteoblast maturation, the protein becomes phosphorylated and is exported to the extracellular matrix, where it orchestrates mineralized matrix formation. Mutations in the gene are known to cause autosomal recessive hypophosphatemia, a disease that manifests as rickets and osteomalacia. The gene structure is conserved in mammals.

    基因ID

    13406

    NCBI登录号

    NP_058059

    UniProt

    O55188
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