CD22 ELISA 试剂盒
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北京 101111
Quick Overview for CD22 ELISA 试剂盒 (ABIN6720081)
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See all CD22 ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 156 pg/mL
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Supplier Product No.
- koa0777
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Supplier
- Rockland
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原理
- Mouse CD22 Sandwich ELISA Kit for Quantitative Detection
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品牌
- AccuSignal™
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Analytical Method
- Quantitative
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特异性
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Expression system for standard: NS0
Immunogen sequence: S22-R702 -
交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- < 10 pg/mL
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组件
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- Antibody-coated 96-well plate
- Target Protein Standard
- Detection antibody
- Detection reagent
- Diluent buffers
- Wash buffers
- Substrate Solution
- Stop solutions
- Adhesive covers
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试剂未包括
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.
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CD22 Molecule (CD22) ELISA Kit
CD22 适用: 人 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
CD22 Molecule (CD22) ELISA KitCD22 适用: 人 Colorimetric Sandwich ELISA 3.12 ng/mL - 200 ng/mL Plasma, Serum
CD22 Molecule (CD22) ELISA KitCD22 适用: 人 Colorimetric Sandwich ELISA 93.8 pg/mL - 6000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
CD22 Molecule (CD22) ELISA KitCD22 适用: 小鼠 AA 22-702 Colorimetric Sandwich ELISA 156 pg/mL - 10000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma (EDTA), Plasma (heparin), Serum
CD22 Molecule (CD22) ELISA KitCD22 适用: 人 Colorimetric Sandwich ELISA 3.12 ng/mL - 200 ng/mL Plasma, Serum
CD22 Molecule (CD22) ELISA KitCD22 适用: 人 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
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说明
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Capture antibody and Detection antibody: polyclonal antibody from goat, polyclonal antibody from goat
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样本量
- 100 μL
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板类型
- Pre-coated
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实验流程
- Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL mouse CD22 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernatants, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each mouse CD22 standard solution and each sample be measured in duplicate.
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实验精密度
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Intra-Assay Precision (CV%): 6.9%, 6%, 4.5%
Inter-Assay Precision (CV%): 7.7%, 6.9%, 5.5% -
限制
- 仅限研究用
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
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有效期
- 12 months
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- CD22 (CD22 Molecule (CD22))
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别名
- CD22
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背景
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Background: CD22 or cluster of differentiation-22, is a molecule belonging to the SIGLEC family of lectins. This gene is mapped to 19q13.2. It is found on the surface of mature B cells and to a lesser extent on some immature B cells. Generally speaking, CD22 is a regulatory molecule that prevents the overactivation of the immune system and the development of autoimmune diseases. It is a negative regulator of antigen receptor signaling whose onset of expression at the mature B cell stage may serve to raise the antigen concentration threshold required for B cell triggering. CD22 functions as an inhibitory receptor for B cell receptor (BCR) signalling. This gene can downmodulate signaling through the IgM and IgD B-cell receptors (BCRs), but not through the IgG BCR, because the IgG cytoplasmic tail prevents CD22 phosphorylation and actually enhances IgG-BCR signaling.
Gene Full Name: CD22 antigen
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基因ID
- 12483
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NCBI登录号
- NP_001036782
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UniProt
- P35329
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