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ANGPTL3 ELISA 试剂盒

This 小鼠 ANGPTL3 ELISA Kit is a Colorimetric ELISA Kit designed to quantify 小鼠 ANGPTL3.
Rockland
产品编号 ABIN6720079
Supplier Product No.: koa0816
发货至: 中国

Quick Overview for ANGPTL3 ELISA 试剂盒 (ABIN6720079)

抗原

See all ANGPTL3 ELISA试剂盒
ANGPTL3 (Angiopoietin-Like 3 (ANGPTL3))

适用

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小鼠

检测方法

Colorimetric

实验类型

Sandwich ELISA

检测范围

156 pg/mL - 10000 pg/mL

应用范围

ELISA

样品类型

Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
  • 最低检测浓度

    156 pg/mL

    Supplier Product No.

    koa0816

    Supplier

    Rockland

    原理

    Mouse ANGPTL3 Sandwich ELISA Kit for Quantitative Detection

    品牌

    AccuSignal™

    Analytical Method

    Quantitative

    特异性

    Production: Natural and recombinant mouse ANGPTL3. There is no detectable cross-reactivity with other relevant proteins.

    灵敏度

    < 10 pg/mL

    组件

    • Antibody-coated 96-well plate
    • Target Protein Standard
    • Detection antibody
    • Detection reagent
    • Diluent buffers
    • Wash buffers
    • Substrate Solution
    • Stop solutions
    • Adhesive covers
  • 应用备注

    Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL mouse ANGPTL3 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each mouse ANGPTL3 standard solution and each sample be measured in duplicate.

    说明

    Standard: Expression system for standard: sf21, Immunogen sequence: S17-T455

    样本量

    100 μL

    板类型

    Pre-coated

    限制

    仅限研究用
  • 储存条件

    4 °C,-20 °C

    储存方法

    Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

    有效期

    12 months
  • 抗原 See all ANGPTL3 ELISA试剂盒

    ANGPTL3 (Angiopoietin-Like 3 (ANGPTL3))

    别名

    ANGPTL3

    背景

    Synonyms: ANG 5, ANG-5, ANG5, Angiopoietin 5, Angiopoietin like 3, Angiopoietin related protein 3, Angiopoietin-5, Angiopoietin-like protein 3, Angiopoietin-related protein 3, ANGL3_HUMAN, ANGPT5, ANGPTL3, ANL3, FHBL2, OTTHUMP00000010719, UNQ153/PRO179

    Background: ANGPTL3, also known as Angiopoietin-like 3 or HYPLl, is a protein that in humans is encoded by the ANGPTL3 gene. It is mapped to 1p31.3. The protein encoded by this gene is a member of the angiopoietin-like family of secreted factors. It is expressed predominantly in the liver, and has the characteristic structure of angiopoietins, consisting of a signal peptide, N-terminal coiled-coil domain, and the C-terminal fibrinogen (FBN)-like domain. ANGPTL3 also acts as dual inhibitor of lipoprotein lipase (LPL) and endothelial lipase (EL), and increases plasma triglyceride and HDL cholesterol in rodents. It can inhibit endothelial lipase to catalyze HDL-phospholipid and increase HDL-PL levels.

    基因ID

    30924

    NCBI登录号

    NP_038941

    UniProt

    Q9R182
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