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ADAM12 ELISA 试剂盒

ADAM12 适用: 小鼠 Colorimetric Sandwich ELISA 156 pg/mL - 10000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
产品编号 ABIN6720076
发货至: 中国
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    ADAM12 (ADAM Metallopeptidase Domain 12 (ADAM12))
    适用
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    小鼠
    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    检测范围
    156 pg/mL - 10000 pg/mL
    最低检测浓度
    156 pg/mL
    应用范围
    ELISA
    原理
    Sandwich ELISA for Quantitative Detection of Antigen
    样品类型
    Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
    Analytical Method
    Quantitative
    产品特性

    Synonyms: ADA12_HUMAN, ADAM 12, ADAM metallopeptidase domain 12, ADAM12, Disintegrin and metalloproteinase domain-containing protein 12, MCMP, MCMPMltna, Meltrin-alpha, MLTN, MLTNA

    Background: ADAM12(Disintegrin and metalloproteinase domain-containing protein 12), also known as MLTN, is an enzyme that in humans is encoded by the ADAM12 gene. This gene encodes a member of the ADAM (a disintegrin and metalloprotease) protein family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. This gene has two alternatively spliced transcripts: a shorter secreted form and a longer membrane-bound form. The shorter form is found to stimulate myogenesis. By RT-PCR and immunoblot analyses that expression of mouse Adam12 increases during muscle regeneration, while the levels of other ADAMs remain constant. Immunofluorescence analysis revealed staining of small, newly formed muscle fibers in regenerating but not normal adult muscle cells. By using of fluorescence in situ hybridization, the ADAM12 gene is mapped to human chromosome 10q26.3.

    Gene Name: ADAM12

    Production: Natural and recombinant mouse ADAM12. There is no detectable cross-reactivity with other relevant proteins.

    Standard: Expression system for standard: CHO, Immunogen sequence: R29-S513

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  • 应用备注

    Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL mouse ADAM12 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernatants, serum or plasma(heparin) to each empty well. It is recommended that each mouse ADAM12 standard solution and each sample be measured in duplicate.

    Blood Product Anticoagulant: Heparin Sodium

    ELISA Dilution: 156pg/mL-10,000pg/mL

    样本量
    100 μL
    板类型
    Pre-coated
    限制
    仅限研究用
  • 储存条件
    RT,4 °C,-20 °C
    储存方法
    Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
  • 抗原 See all ADAM12 ELISA试剂盒
    ADAM12 (ADAM Metallopeptidase Domain 12 (ADAM12))
    别名
    ADAM12 (ADAM12 产品)
    别名
    ADAM12 ELISA Kit, adam12 ELISA Kit, Mltna ELISA Kit, mKIAA4001 ELISA Kit, MCMP ELISA Kit, MCMPMltna ELISA Kit, MLTN ELISA Kit, MLTNA ELISA Kit, ADAM metallopeptidase domain 12 ELISA Kit, a disintegrin and metallopeptidase domain 12 (meltrin alpha) ELISA Kit, ADAM12 ELISA Kit, adam12 ELISA Kit, Adam12 ELISA Kit
    基因ID
    11489
    NCBI登录号
    NP_031426
    UniProt
    Q61824
    途径
    EGFR Signaling Pathway
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