SLAMF1 ELISA 试剂盒
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Quick Overview for SLAMF1 ELISA 试剂盒 (ABIN6720073)
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检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 156 pg/mL
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Supplier Product No.
- koa0793
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Supplier
- Rockland
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原理
- Human SLAM - CD150 Sandwich ELISA Kit for Quantitative Detection
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品牌
- AccuSignal™
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Analytical Method
- Quantitative
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特异性
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Expression system for standard: NS0
Immunogen sequence: A21-K236 -
交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- < 10 pg/mL
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组件
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- Antibody-coated 96-well plate
- Target Protein Standard
- Detection antibody
- Detection reagent
- Diluent buffers
- Wash buffers
- Substrate Solution
- Stop solutions
- Adhesive covers
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试剂未包括
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.
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Signaling Lymphocytic Activation Molecule Family Member 1 (SLAMF1) ELISA Kit
SLAMF1 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Signaling Lymphocytic Activation Molecule Family Member 1 (SLAMF1) ELISA KitSLAMF1 适用: 人 AA 21-236 Colorimetric Sandwich ELISA 156 pg/mL - 10000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma (EDTA), Plasma (heparin), Serum
Signaling Lymphocytic Activation Molecule Family Member 1 (SLAMF1) ELISA KitSLAMF1 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
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说明
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Capture antibody and Detection antibody: monoclonal antibody from mouse, polyclonal antibody from goat
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样本量
- 100 μL
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板类型
- Pre-coated
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实验流程
- Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human SLAM standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human SLAM standard solution and each sample be measured in duplicate.
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实验精密度
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Intra-Assay Precision (CV%): 4.9%, 4.9%, 5%
Inter-Assay Precision (CV%): 6%, 5.5%, 5% -
限制
- 仅限研究用
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
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有效期
- 12 months
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- SLAMF1 (Signaling Lymphocytic Activation Molecule Family Member 1 (SLAMF1))
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别名
- SLAM/CD150
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背景
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Background: Signaling lymphocytic activation molecule is a protein that in humans is encoded by the SLAMF1 gene. It belongs to the immunoglobulin gene superfamily. This gene is mapped to 1q23.3. It has found that SLAM is constitutively expressed on peripheral blood memory T cells, T-cell clones, immature thymocytes and a proportion of B cells, and is rapidly induced on naive T cells after activation. In MV-resistant cell lines, infection with clinical MV and expression of SLAM, but not CD46, caused cytopathic effects (CPE). The expression of SLAM on activated B and T lymphocytes correlates with the pathology of MV infection in humans and monkeys, in which lymphoid organs are the chief sites of MV replication and the binding of MV to SLAM may impair the signaling functions of SLAM in lymphocyte activation and inhibit Th0/Th1 cytokine production, thereby promoting Th2 cytokine production. It has reported that antibody-mediated ligation of SLAM on thymocytes triggered a protein tyrosine phosphorylation signal in T cells in a SAP-dependent manner. This signal also involved SHIP, the adaptor molecules DOK2, DOK1, and SHC and RASGAP.
Gene Full Name: signaling lymphocytic activation molecule family member 1
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基因ID
- 6504
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NCBI登录号
- NP_003028
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UniProt
- Q13291
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