IL-27 ELISA 试剂盒
Quick Overview for IL-27 ELISA 试剂盒 (ABIN6720070)
抗原
See all IL-27 (IL27) ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 15.6 pg/mL
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Supplier Product No.
- koa0473
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Supplier
- Rockland
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原理
- Mouse IL-27 p28 Sandwich ELISA Kit for Quantitative Detection
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品牌
- AccuSignal™
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Analytical Method
- Quantitative
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特异性
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Production: Natural and recombinant mouse IL-27 p28. There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- < 10 pg/mL
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组件
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- Antibody-coated 96-well plate
- Target Protein Standard
- Detection antibody
- Detection reagent
- Diluent buffers
- Wash buffers
- Substrate Solution
- Stop solutions
- Adhesive covers
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Interleukin 27 (IL27) ELISA Kit
IL27 适用: 小鼠 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
Interleukin 27 (IL27) ELISA KitIL27 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 27 (IL27) ELISA KitIL27 适用: 大鼠 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 27 (IL27) ELISA KitIL27 适用: 小鼠 Colorimetric Sandwich ELISA 7.813 pg/mL - 500 pg/mL Plasma, Serum, Tissue Homogenate
Interleukin 27 (IL27) ELISA KitIL27 适用: 绵羊 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Interleukin 27 (IL27) ELISA KitIL27 适用: 小鼠 AA 29-234 Colorimetric Sandwich ELISA 15.6-1000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Interleukin 27 (IL27) ELISA KitIL27 适用: 人 AA 21-229 Colorimetric Sandwich ELISA 156-10000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Interleukin 27 (IL27) ELISA KitIL27 适用: 小鼠 Colorimetric Sandwich ELISA 16-1000 pg/mL Cell Lysate, Plasma, Serum
Interleukin 27 (IL27) ELISA KitIL27 适用: 人 Colorimetric Sandwich ELISA 156 pg/mL - 10000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
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应用备注
- Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL mouse IL-27 p28 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each mouse IL-27 p28 standard solution and each sample be measured in duplicate.
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说明
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Standard: Expression system for standard: NSO, Immunogen sequence: F29-S234
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样本量
- 100 μL
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板类型
- Pre-coated
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限制
- 仅限研究用
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
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有效期
- 12 months
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- IL-27 (IL27) (Interleukin 27 (IL27))
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别名
- IL-27 p28
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背景
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Synonyms: IL 27A, IL 30, IL-27 subunit alpha, IL-27-A, IL27, IL27-A, IL27A, IL27A_HUMAN, IL27p28, IL30, Interleukin 27, Interleukin 27 subunit alpha, Interleukin 30, Interleukin-27 subunit alpha, p29
Background: Interleukin-27(IL-27) is a heterodimeric cytokine belonging to the IL-12 family that is composed of two subunits, Epstein-Barr virus(EBV)-induced gene 3(EBI3)(also known as IL-27B) and IL27-p28(known as IL-30). IL-27 is produced by antigen-presenting cells. By genomic sequence analysis, the IL27 p28 gene was mapped to chromosome 16p11. L-27 plays an important function in regulating the activity of B- and T-lymphocytes. The effects of IL-27 are eliciting by its interaction with a specific cell-surface receptor complex composed of two proteins known as IL27R and gp130.
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基因ID
- 246779
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NCBI登录号
- NP_663611
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UniProt
- Q8K3I6
抗原 See all IL-27 (IL27) ELISA试剂盒
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