GDF15 ELISA 试剂盒
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北京 101111
Quick Overview for GDF15 ELISA 试剂盒 (ABIN6720065)
抗原
See all GDF15 ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 15.6 pg/mL
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Supplier Product No.
- koa0649
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Supplier
- Rockland
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原理
- Mouse GDF15 Sandwich ELISA Kit for Quantitative Detection
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品牌
- AccuSignal™
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Analytical Method
- Quantitative
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特异性
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Expression system for standard: NS0
Immunogen sequence: S189-A303 -
交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- < 10 pg/mL
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组件
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- Antibody-coated 96-well plate
- Target Protein Standard
- Detection antibody
- Detection reagent
- Diluent buffers
- Wash buffers
- Substrate Solution
- Stop solutions
- Adhesive covers
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试剂未包括
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.
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Growth Differentiation Factor 15 (GDF15) ELISA Kit
GDF15 适用: 人 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Plasma, Serum, Tissue Homogenate, Urine
Growth Differentiation Factor 15 (GDF15) ELISA KitGDF15 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Growth Differentiation Factor 15 (GDF15) ELISA KitGDF15 适用: 人 Colorimetric Sandwich ELISA 23.44 pg/mL - 1500 pg/mL Cell Culture Supernatant, Plasma, Serum
Growth Differentiation Factor 15 (GDF15) ELISA KitGDF15 适用: 大鼠 Colorimetric Competition ELISA 1.56 pg/mL - 100 pg/mL Plasma, Serum, Tissue Homogenate
Growth Differentiation Factor 15 (GDF15) ELISA KitGDF15 适用: 小鼠 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
Growth Differentiation Factor 15 (GDF15) ELISA KitGDF15 适用: 人 Colorimetric Sandwich ELISA 23.438 pg/mL - 1500 pg/mL Plasma, Serum, Tissue Homogenate
Growth Differentiation Factor 15 (GDF15) ELISA KitGDF15 适用: 小鼠 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Growth Differentiation Factor 15 (GDF15) ELISA KitGDF15 适用: 大鼠 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Growth Differentiation Factor 15 (GDF15) ELISA KitGDF15 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Growth Differentiation Factor 15 (GDF15) ELISA KitGDF15 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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说明
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Capture antibody and Detection antibody: polyclonal antibody from sheep, polyclonal antibody from sheep
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样本量
- 100 μL
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板类型
- Pre-coated
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实验流程
- Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.3pg/mL, 15.6pg/mL mouse GDF-15 standard solutions into the pre-coated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. We recommend that each mouse GDF-15 standard solution and each sample is measured in duplicate.
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实验精密度
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Intra-Assay Precision (CV%): 4.9%, 4.3%, 6%
Inter-Assay Precision (CV%): 6%, 6%, 6.6% -
限制
- 仅限研究用
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
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有效期
- 12 months
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- GDF15 (Growth Differentiation Factor 15 (GDF15))
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别名
- GDF-15
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背景
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Background: GDF-15 (Growth differentiation factor 15),also known as TGF-PL, MIC-1, PDF, PLAB, and PTGFB, is a protein belonging to the transforming growth factor beta superfamily that has a role in regulating inflammatory and apoptotic pathways in injured tissues and during disease processes. Using FISH, the MIC1 gene is mapped to 19p13.2-p13.1. Its expression in liver can be significantly up-regulated in during injury of organs such as liver, kidney, heart and lung. GDF15 showed increased expression and secretion during erythroblast maturation. GDF15 functions as an antiinflammatory cytokine by ly interfering with chemokine signaling and integrin activation.
Gene Full Name: growth differentiation factor 15
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基因ID
- 23886
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NCBI登录号
- NP_035949
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UniProt
- Q9Z0J7
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途径
- SARS-CoV-2 Protein Interactome
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