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IL-15 ELISA 试剂盒

This 人 IL-15 ELISA Kit is a Colorimetric ELISA Kit designed to quantify 人 IL-15.
Rockland
产品编号 ABIN6720049
Supplier Product No.: koa0239
发货至: 中国

Quick Overview for IL-15 ELISA 试剂盒 (ABIN6720049)

抗原

See all IL-15 (IL15) ELISA试剂盒
IL-15 (IL15) (Interleukin 15 (IL15))

适用

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检测方法

Colorimetric

实验类型

Sandwich ELISA

检测范围

15.6 pg/mL - 1000 pg/mL

应用范围

ELISA

样品类型

Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
  • 最低检测浓度

    15.6 pg/mL

    Supplier Product No.

    koa0239

    Supplier

    Rockland

    原理

    Human IL-15 Sandwich ELISA Kit for Quantitative Detection

    品牌

    AccuSignal™

    Analytical Method

    Quantitative

    特异性

    Production: Natural and recombinant human IL-15. There is no detectable cross-reactivity with other relevant proteins.

    灵敏度

    < 3 pg/mL

    组件

    • Antibody-coated 96-well plate
    • Target Protein Standard
    • Detection antibody
    • Detection reagent
    • Diluent buffers
    • Wash buffers
    • Substrate Solution
    • Stop solutions
    • Adhesive covers
  • 应用备注

    Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.3pg/mL, 15.6pg/mL human IL-15 standard solutions into the pre-coated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. We recommend that each human IL-15 standard solution and each sample is measured in duplicate.

    说明

    Standard: Expression system for standard: E.coli, Immunogen sequence: N49-S162

    样本量

    100 μL

    板类型

    Pre-coated

    限制

    仅限研究用
  • 储存条件

    4 °C,-20 °C

    储存方法

    Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

    有效期

    12 months
  • 抗原 See all IL-15 (IL15) ELISA试剂盒

    IL-15 (IL15) (Interleukin 15 (IL15))

    别名

    IL-15

    背景

    Synonyms: IL 15, Interleukin 15, Interleukin15, MGC9721

    Background: Interleukin(IL)-15 is a cytokine with the ability to stimulate the proliferation activity of Th1 and/or Th2 lymphocytes. IL-15 is a novel cytokine whose effects on T-cell activation and proliferation are similar to those of interleukin-2(IL-2), presumably because IL-15 utilizes the beta and gamma chains of the IL-2 receptor. IL-15 can play a role in the initiation and outcome of acute and chronic rejection. Anti-IL-15 therapy in combination with classic immunosuppression therapy might thus be beneficial in the prevention of acute, and especially chronic, allograft rejection. The human IL15 gene is mapped to human chromosome 4q31 by fluorescence in situ hybridization.1 The standard product used in this kit is recombinant human IL-15, consisting of 114 amino acids with the molecular mass of 12.9KDa.

    基因ID

    3600

    NCBI登录号

    NP_000576

    UniProt

    P40933

    途径

    JAK/STAT Signaling, Glycosaminoglycan Metabolic Process
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