Prokineticin 1 ELISA 试剂盒
Quick Overview for Prokineticin 1 ELISA 试剂盒 (ABIN6720042)
抗原
See all Prokineticin 1 (Prok1) ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 15.6 pg/mL
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Supplier Product No.
- koa0351
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Supplier
- Rockland
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原理
- Human EG-VEGF Sandwich ELISA Kit for Quantitative Detection
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品牌
- AccuSignal™
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Analytical Method
- Quantitative
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特异性
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Production: Natural and recombinant human EG-VEGF. There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- < 7 pg/mL
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组件
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- Antibody-coated 96-well plate
- Target Protein Standard
- Detection antibody
- Detection reagent
- Diluent buffers
- Wash buffers
- Substrate Solution
- Stop solutions
- Adhesive covers
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Prokineticin 1 (Prok1) ELISA Kit
Prok1 适用: 小鼠 Colorimetric Sandwich ELISA 15.62 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Prokineticin 1 (Prok1) ELISA KitProk1 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Prokineticin 1 (Prok1) ELISA KitProk1 适用: 大鼠 Colorimetric Sandwich ELISA 15.62 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Prokineticin 1 (Prok1) ELISA KitProk1 适用: Cow Colorimetric Sandwich ELISA 15.62 pg/mL - 1000 pg/mL Plasma, Serum
Prokineticin 1 (Prok1) ELISA KitProk1 适用: 人 AA 20-105 Colorimetric Sandwich ELISA 15.6-1000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
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应用备注
- Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL human EG-VEGF standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human EG-VEGF standard solution and each sample be measured in duplicate.
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说明
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Standard: Expression system for standard: NSO, Immunogen sequence: A20-F105
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样本量
- 100 μL
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板类型
- Pre-coated
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限制
- 仅限研究用
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
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有效期
- 12 months
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- Prokineticin 1 (Prok1)
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别名
- EG-VEGF
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背景
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Synonyms: EG-VEGF, Endocrine-gland-derived vascular endothelial growth factor, Mambakine, PROK1, PROK1_HUMAN, Prokineticin-1
Background: EG-VEGF, Endocrine gland derived vascular endothelial growth factor, is a human protein encoded by the PROKR1 gene. Its gene is mapped to chromosome 1. The 1.4-kb cDNA encodes a protein of 105 amino acids with a well defined signal sequence. The mature protein is predicted to have 86 amino acids. Northern blot analysis demonstrated expression in testis, ovary, adrenal gland, and placenta. EG-VEGF has played a vital role in inducing proliferation, migration, and fenestration in capillary endothelial cells derived from endocrine glands. The standards of this kit are recombinant human EG-VEGF(A20-F105), with molecular weight of 10.5 KDa.
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基因ID
- 84432
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NCBI登录号
- NP_115790
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UniProt
- P58294
抗原 See all Prokineticin 1 (Prok1) ELISA试剂盒
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