CCL15 ELISA 试剂盒
Quick Overview for CCL15 ELISA 试剂盒 (ABIN6720038)
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See all CCL15 ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 15.6 pg/mL
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Supplier Product No.
- koa0640
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Supplier
- Rockland
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原理
- Human CCL15 Sandwich ELISA Kit for Quantitative Detection
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品牌
- AccuSignal™
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Analytical Method
- Quantitative
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特异性
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Production: Natural and recombinant human CCL15. There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- < 10 pg/mL
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组件
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- Antibody-coated 96-well plate
- Target Protein Standard
- Detection antibody
- Detection reagent
- Diluent buffers
- Wash buffers
- Substrate Solution
- Stop solutions
- Adhesive covers
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Chemokine (C-C Motif) Ligand 15 (CCL15) ELISA Kit
CCL15 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Chemokine (C-C Motif) Ligand 15 (CCL15) ELISA KitCCL15 适用: 人 AA 22-113 Colorimetric Sandwich ELISA 15.6-1000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Chemokine (C-C Motif) Ligand 15 (CCL15) ELISA KitCCL15 适用: 大鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Plasma, Serum
Chemokine (C-C Motif) Ligand 15 (CCL15) ELISA KitCCL15 适用: 小鼠 Colorimetric Sandwich ELISA 0.16 ng/mL - 10 ng/mL Plasma, Serum
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应用备注
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ELISA_Dilution: 15.6pg/mL-1000pg/mL
Application_Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL human CCL15 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human CCL15 standard solution and each sample be measured in duplicate.
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说明
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Standard: Expression system for standard: E.coli, Immunogen sequence: Q22-I113
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样本量
- 100 μL
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板类型
- Pre-coated
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限制
- 仅限研究用
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
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有效期
- 12 months
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- CCL15 (Chemokine (C-C Motif) Ligand 15 (CCL15))
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别名
- CCL15
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背景
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Synonyms: CC chemokine 3, CCL15, CCL15(29-92), CCL15_HUMAN, Chemokine CC-2, Chemokine CC2, HCC-2, HCC2, HMRP 2B, HMRP2B, Leukotactin 1, Leukotactin-1, LKN-1, LKN1, Macrophage inflammatory protein 5, MIP-1 delta, MIP-5, MIP1 delta, MIP1d, MIP5, Mrp-2b, Mrp2b, NCC-3, NCC3, SCYA15, SCYL3, Small inducible cytokine A15, Small inducible cytokine subfamily A (Cys-Cys), member 15, Small-inducible cytokine A15, SY15
Background: Chemokine(C-C motif) ligand 15(CCL15) is a small cytokine belonging to the CC chemokine family that is also known as leukotactin-1, MIP5 and HCC-2. CCL15 is expressed in liver, small intestine, colon, and in certain leukocytes and macrophages of the lung. It is chemotactic for neutrophils, monocytes, and lymphocytes and elicits its effects by binding to cell surface chemokine receptors like CCR1 and CCR3. The human CCL15 gene spans four exons and is located in a head-to-tail orientation on chromosome 17 with the gene of another CC chemokine known as CCL14.
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基因ID
- 6359
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NCBI登录号
- NP_116741
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UniProt
- Q16663
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