N-Cadherin ELISA 试剂盒
Quick Overview for N-Cadherin ELISA 试剂盒 (ABIN6720008)
抗原
See all N-Cadherin (CDH2) ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 0.78 ng/mL
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Supplier Product No.
- koa0384
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Supplier
- Rockland
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原理
- Human Cadherin-2 - N-Cadherin Sandwich ELISA Kit for Quantitative Detection
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品牌
- AccuSignal™
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Analytical Method
- Quantitative
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特异性
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Production: Natural and recombinant human Cadherin-2. There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- < 20 pg/mL
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组件
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- Antibody-coated 96-well plate
- Target Protein Standard
- Detection antibody
- Detection reagent
- Diluent buffers
- Wash buffers
- Substrate Solution
- Stop solutions
- Adhesive covers
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Cadherin 2 (CDH2) ELISA Kit
CDH2 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Cadherin 2 (CDH2) ELISA KitCDH2 适用: 大鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Cadherin 2 (CDH2) ELISA KitCDH2 适用: 小鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Cadherin 2 (CDH2) ELISA KitCDH2 适用: 小鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
Cadherin 2 (CDH2) ELISA KitCDH2 适用: 犬 AA 160-724 Colorimetric Sandwich ELISA
Cadherin 2 (CDH2) ELISA KitCDH2 适用: 兔 AA 160-724 Colorimetric Sandwich ELISA
Cadherin 2 (CDH2) ELISA KitCDH2 适用: 小鼠 AA 160-724 Colorimetric Sandwich ELISA 0.78-50 ng/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Cadherin 2 (CDH2) ELISA KitCDH2 适用: 大鼠 AA 160-724 Colorimetric Sandwich ELISA 0.78-50 ng/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Cadherin 2 (CDH2) ELISA KitCDH2 适用: 人 AA 160-724 Colorimetric Sandwich ELISA 0.78-50 ng/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Cadherin 2 (CDH2) ELISA KitCDH2 适用: 猴 AA 160-724 Colorimetric Sandwich ELISA
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应用备注
- Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 50 ng/mL, 25 ng/mL, 12.5 ng/mL, 6.25 ng/mL, 3.12 ng/mL, 1.56 ng/mL, 0.78 ng/mL human Cadherin-2 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. We recommend that each human Cadherin-2 standard solution and each sample is measured in duplicate.
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说明
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Standard: Expression system for standard: NSO, Immunogen sequence: D160-A724
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样本量
- 100 μL
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板类型
- Pre-coated
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限制
- 仅限研究用
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
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有效期
- 12 months
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- N-Cadherin (CDH2) (Cadherin 2 (CDH2))
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别名
- Cadherin-2/N-Cadherin
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背景
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Synonyms: CDw325, CDHN, Neural cadherin, N-cadherin, CD325
Background: Cadherin-2(CDH2), also known as neural cadherin(NCAD), is a protein that in humans is encoded by the CDH2 gene. It is a classical cadherin from the cadherin superfamily. This gene is mapped to 18q12.1. Cadherin-2 is expressed in the brain, skeletal and cardiac muscle. Cadherin-2 is commonly found in cancer cells and provides a mechanism for transendothelial migration. It is a calcium dependent cell-cell adhesion glycoprotein comprising five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. The protein functions during gastrulation and is required for establishment of left-right asymmetry. At certain central nervous system synapses, presynaptic to postsynaptic adhesion is mediated at least in part by this gene product.
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基因ID
- 1000
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NCBI登录号
- NP_001295105
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UniProt
- P19022
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途径
- Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Synaptic Membrane
抗原 See all N-Cadherin (CDH2) ELISA试剂盒
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