我们推荐在您的浏览器上开启Javascript，以获得最佳效果。

IL12B ELISA 试剂盒

Name: IL12B ELISA 试剂盒
SKU: ABIN6719989
Availability: OutOfStock

This 小鼠 IL12B ELISA Kit is a Colorimetric ELISA Kit designed to quantify 小鼠 IL12B.

ELISA

1 image

产品编号 ABIN6719989

Supplier Product No.: koa0567

发货至: 中国

Quick Overview for IL12B ELISA 试剂盒 (ABIN6719989)

抗原

See all IL12B ELISA试剂盒

IL12B (Interleukin 12 beta (IL12B))

适用

小鼠

检测方法

Colorimetric

实验类型

Sandwich ELISA

检测范围

15.6 pg/mL - 1000 pg/mL

应用范围

ELISA

样品类型

Cell Culture Supernatant, Plasma (EDTA - heparin - citrate), Serum

Discover our top product IL12B ELISA Kit

最低检测浓度

15.6 pg/mL

Supplier Product No.

koa0567

Supplier

Rockland

原理

Mouse IL-12(p40) Sandwich ELISA Kit for Quantitative Detection

品牌

AccuSignal™

Analytical Method

Quantitative

特异性

Production: Natural and recombinant mouse IL-12(p40). There is no detectable cross-reactivity with other relevant proteins.

灵敏度

< 10 pg/mL

组件
- Antibody-coated 96-well plate
- Target Protein Standard
- Detection antibody
- Detection reagent
- Diluent buffers
- Wash buffers
- Substrate Solution
- Stop solutions
- Adhesive covers
Interleukin 12 beta (IL12B) ELISA Kit
IL12B 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Interleukin 12 beta (IL12B) ELISA Kit
IL12B 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Interleukin 12 beta (IL12B) ELISA Kit
IL12B 适用: 马 Colorimetric Sandwich ELISA 15.62 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Interleukin 12 beta (IL12B) ELISA Kit
IL12B 适用: 小鼠 AA 23-335 Colorimetric Sandwich ELISA 15.6-1000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum

Interleukin 12 beta (IL12B) ELISA Kit
IL12B 适用: 人 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate, Urine

Interleukin 12 beta (IL12B) ELISA Kit
IL12B 适用: 绵羊 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate

Interleukin 12 beta (IL12B) ELISA Kit
IL12B 适用: 山羊 Colorimetric Sandwich ELISA 9.38 pg/mL - 600 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate

Interleukin 12 beta (IL12B) ELISA Kit
IL12B 适用: 人 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Interleukin 12 beta (IL12B) ELISA Kit
High Sensitivity IL12B 适用: Cow Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Interleukin 12 beta (IL12B) ELISA Kit
IL12B 适用: 小鸡 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

应用备注

Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL mouse IL-12(p40) standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA, citrate) to each empty well. It is recommended that each mouse IL-12(p40) standard solution and each sample be measured in duplicate.

说明

Standard: Expression system for standard: sf21, Immunogen sequence: M23-S335

样本量

100 μL

板类型

Pre-coated

限制

仅限研究用
储存条件

4 °C,-20 °C

储存方法

Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

有效期

12 months
抗原 See all IL12B ELISA试剂盒

IL12B (Interleukin 12 beta (IL12B))

别名

IL-12(p40)

背景

Synonyms: CLMF, CLMF p40, CLMF2, Cytotoxic lymphocyte maturation factor 40 kDa subunit, IL 12B, IL-12 subunit p40, IL-12B, IL12 p40, IL12B, IL12B_HUMAN, Interleukin 12 subunit beta, NK cell stimulatory factor chain 2, NKSF, NKSF2
Background: Interleukin-12(IL12, formerly NKSF, for natural killer cell stimulatory factor, or CLMF, for cytotoxic lymphocyte maturation factor) is a novel cytokine cloned from B-cell lines. IL-12 is a heterodimeric molecule composed of p35 and p40 subunits.1 The larger 40- kDa subunit(p40) is a member of the cytokine receptor family, and the smaller 35- kDa subunit(p35) is related to IL6 and GCSF.2 Both IL-12 p40(-/-) and p35(-/-) mice fail to produce IL-12 p70 heterodimer.3 Interleukin(IL)-12 has been cloned on the basis of its ability to activate natural killer(NK) cells and promote the development of cytolytic T cells. With further understanding of its activities, IL-12 has emerged as an important cytokine, affecting both immune and hematologic functions. It has been shown to be necessary for the T cell independent induction of interferon(IFN)-gamma, critical for the initial suppression of bacterial and parasitic infection, for the development of a Th1 response, critical for effective host defense against intracellular pathogens, and for the activation of differentiated T lymphocytes of both CD4+ and CD8+ phenotype.

基因ID

16160

NCBI登录号

NP_001290173

UniProt

P43432

途径

JAK/STAT Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation