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CXCL5 ELISA 试剂盒

This 大鼠 CXCL5 ELISA Kit is a Colorimetric ELISA Kit designed to quantify 大鼠 CXCL5.
Rockland
产品编号 ABIN6719966
Supplier Product No.: koa0491
发货至: 中国

Quick Overview for CXCL5 ELISA 试剂盒 (ABIN6719966)

抗原

See all CXCL5 ELISA试剂盒
CXCL5 (Chemokine (C-X-C Motif) Ligand 5 (CXCL5))

适用

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大鼠

检测方法

Colorimetric

实验类型

Sandwich ELISA

检测范围

15.6 pg/mL - 1000 pg/mL

应用范围

ELISA

样品类型

Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
  • 最低检测浓度

    15.6 pg/mL

    Supplier Product No.

    koa0491

    Supplier

    Rockland

    原理

    Rat CXCL5 - LIX Sandwich ELISA Kit for Quantitative Detection

    品牌

    AccuSignal™

    Analytical Method

    Quantitative

    特异性

    Production: Natural and recombinant rat CXCL5. There is no detectable cross-reactivity with other relevant proteins.

    灵敏度

    < 1 pg/mL

    组件

    • Antibody-coated 96-well plate
    • Target Protein Standard
    • Detection antibody
    • Detection reagent
    • Diluent buffers
    • Wash buffers
    • Substrate Solution
    • Stop solutions
    • Adhesive covers
  • 应用备注

    Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL rat CXCL5 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of rat cell culture supernatants, serum, plasma(heparin, EDTA, citrate) or tissue homogenates to each empty well. It is recommended that each rat CXCL5 standard solution and each sample be measured in duplicate.

    说明

    Standard: Expression system for standard: E.coli, Immunogen sequence: A38-Q130

    样本量

    100 μL

    板类型

    Pre-coated

    限制

    仅限研究用
  • 储存条件

    4 °C,-20 °C

    储存方法

    Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

    有效期

    12 months
  • 抗原 See all CXCL5 ELISA试剂盒

    CXCL5 (Chemokine (C-X-C Motif) Ligand 5 (CXCL5))

    别名

    CXCL5/LIX

    背景

    Synonyms: AMCFII, C-X-C motif chemokine 5, chemokine (C-X-C motif) ligand 5, Cxcl5, CXCL5_HUMAN, ENA-78 (8-78), ENA-78(1-78), ENA-78(9-78), ENA78, Epithelial derived neutrophil activating peptide 78, Epithelial-derived neutrophil-activating protein 78, GCP2, Lipopolysaccharide-induced CXC chemokine, LIX, Neutrophil activating peptide ENA 78, neutrophil-activating protein 78, SCYB5, Scyb6, Small inducible cytokine B5, Small inducible cytokine subfamily B member 5, Small-inducible cytokine B5

    Background: C-X-C motif chemokine 5 is a protein that in humans encoded by the CXCL5 gene. The protein encoded by this gene, CXCL5 is a small cytokine belonging to the CXC chemokine family that is also known as epithelial-derived neutrophil-activating peptide 78(ENA-78). It is produced following stimulation of cells with the inflammatory cytokines interleukin-1or tumor necrosis factor-alpha. Expression of CXCL5 has also been observed in eosinophils, and can be inhibited with the type II interferon IFN-gamma. This chemokine stimulates the chemotaxis of neutrophils possessing angiogenic properties. It elicits these effects by interacting with the cell surface chemokine receptor CXCR2. The gene for CXCL5 is encoded on four exons and is located on human chromosome 4 amongst several other CXC chemokine genes. CXCL5 has been implicated in connective tissue remodeling. CXCL5 plays a role in reducing sensitivity to sunburn pain in some subjects, and is a potential target which can be utilized to understand more about pain in other inflammatory conditions like arthritis and cystitis.

    基因ID

    60665

    NCBI登录号

    NP_071550

    UniProt

    P97885

    途径

    Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity
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