CCL7 ELISA 试剂盒
Quick Overview for CCL7 ELISA 试剂盒 (ABIN6719954)
抗原
See all CCL7 ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 15.6 pg/mL
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Supplier Product No.
- koa0393
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Supplier
- Rockland
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原理
- Mouse CCL7 - MCP3 Sandwich ELISA Kit for Quantitative Detection
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品牌
- AccuSignal™
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Analytical Method
- Quantitative
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特异性
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Production: Natural and recombinant mouse CCL7. There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- < 1 pg/mL
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组件
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- Antibody-coated 96-well plate
- Target Protein Standard
- Detection antibody
- Detection reagent
- Diluent buffers
- Wash buffers
- Substrate Solution
- Stop solutions
- Adhesive covers
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Chemokine (C-C Motif) Ligand 7 (CCL7) ELISA Kit
CCL7 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Chemokine (C-C Motif) Ligand 7 (CCL7) ELISA KitCCL7 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Chemokine (C-C Motif) Ligand 7 (CCL7) ELISA KitCCL7 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Chemokine (C-C Motif) Ligand 7 (CCL7) ELISA KitSmall Sample CCL7 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Chemokine (C-C Motif) Ligand 7 (CCL7) ELISA KitCCL7 适用: 人 AA 24-99 Colorimetric Sandwich ELISA 15.6-1000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Chemokine (C-C Motif) Ligand 7 (CCL7) ELISA KitCCL7 适用: 大鼠 AA 24-97 Colorimetric Sandwich ELISA 15.6-1000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Chemokine (C-C Motif) Ligand 7 (CCL7) ELISA KitCCL7 适用: 小鼠 AA 24-97 Colorimetric Sandwich ELISA 15.6-1000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Chemokine (C-C Motif) Ligand 7 (CCL7) ELISA KitCCL7 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
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应用备注
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ELISA_Dilution: 15.6pg/mL-1000pg/mL
Application_Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL mouse CCL7 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each mouse CCL7 standard solution and each sample be measured in duplicate.
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说明
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Standard: Expression system for standard: E.coli, Immunogen sequence: Q24-P97
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样本量
- 100 μL
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板类型
- Pre-coated
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限制
- 仅限研究用
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
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有效期
- 12 months
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- CCL7 (Chemokine (C-C Motif) Ligand 7 (CCL7))
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别名
- CCL7/MCP3
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背景
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Synonyms: C-C motif chemokine 7, Ccl7, CCL7_HUMAN, Chemokine CC Motif Ligand 7, Chemotactic protein 3, FIC, Intercrine/chemokine MARC, MARC, MCP 3, MCP-3, Monocyte chemoattractant protein 3, Monocyte chemotactic protein 3, NC28, RANTES/sis homolog, RP23-350G1.4, SCYA6, SCYA7, Small inducible cytokine A7, Small-inducible cytokine A7
Background: Chemokine(C-C motif) ligand 7(CCL7) is a small cytokine known as a chemokine that was previously called monocyte-specific chemokine 3(MCP3). It belongs to the C-C chemokine family. By fluorescence in situ hybridization, mapped the MCP3 gene to chromosome 17q11.2-q12. MCP3 was identified as a physiologic substrate of gelatinase A. Cleaved MCP3 binds to CC-chemokine receptors-1, -2, and -3, but no longer induces calcium fluxes or promotes chemotaxis, and instead acts as a general chemokine antagonist that dampens inflammation, suggested that matrix metalloproteinases are both effectors and regulators of the inflammatory response.
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基因ID
- 20306
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NCBI登录号
- NP_038682
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UniProt
- Q03366
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