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IL-7 ELISA 试剂盒

This 小鼠 IL-7 ELISA Kit is a Colorimetric ELISA Kit designed to quantify 小鼠 IL-7.
Rockland
产品编号 ABIN6719941
Supplier Product No.: koa0460
发货至: 中国

Quick Overview for IL-7 ELISA 试剂盒 (ABIN6719941)

抗原

See all IL-7 (IL7) ELISA试剂盒
IL-7 (IL7) (Interleukin 7 (IL7))

适用

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小鼠

检测方法

Colorimetric

实验类型

Sandwich ELISA

检测范围

15.6 pg/mL - 1000 pg/mL

应用范围

ELISA

样品类型

Cell Culture Supernatant, Plasma (EDTA), Serum
  • 最低检测浓度

    15.6 pg/mL

    Supplier Product No.

    koa0460

    Supplier

    Rockland

    原理

    Mouse IL-7 Sandwich ELISA Kit for Quantitative Detection

    品牌

    AccuSignal™

    Analytical Method

    Quantitative

    特异性

    Production: Natural and recombinant mouse IL-7. There is no detectable cross-reactivity with other relevant proteins.

    灵敏度

    < 1 pg/mL

    组件

    • Antibody-coated 96-well plate
    • Target Protein Standard
    • Detection antibody
    • Detection reagent
    • Diluent buffers
    • Wash buffers
    • Substrate Solution
    • Stop solutions
    • Adhesive covers
  • 应用备注

    ELISA_Dilution: 15.6pg/mL-1000pg/mL

    Application_Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL mouse IL-7 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(EDTA) to each empty well. It is recommended that each mouse IL-7 standard solution and each sample be measured in duplicate.

    说明

    Standard: Expression system for standard: E.coli, Immunogen sequence: E26-I154

    样本量

    100 μL

    板类型

    Pre-coated

    限制

    仅限研究用
  • 储存条件

    4 °C,-20 °C

    储存方法

    Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

    有效期

    12 months
  • 抗原 See all IL-7 (IL7) ELISA试剂盒

    IL-7 (IL7) (Interleukin 7 (IL7))

    别名

    IL-7

    背景

    Synonyms: IL 7, IL7 nirs variant 6, Interleukin 7, Interleukin8

    Background: Interleukin 7(IL7) is a protein that is encoded by the IL7 gene in humans. The gene for human interleukin 7(IL7) maps to chromosome 8 by Southern analysis of a somatic cell hybrid panel and to 8q12-q13 by in situ hybridization.1IL-7 is essential for survival and expansion of pathogenic T helper type 17(T(H)17) cells in experimental autoimmune encephalomyelitis(EAE).2

    基因ID

    16196

    NCBI登录号

    NP_032397

    UniProt

    P10168

    途径

    JAK/STAT Signaling
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