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IL-7 ELISA 试剂盒

This 人 IL-7 ELISA Kit is a Colorimetric ELISA Kit designed to quantify 人 IL-7.
Rockland
产品编号 ABIN6719939
Supplier Product No.: koa0459
发货至: 中国

Quick Overview for IL-7 ELISA 试剂盒 (ABIN6719939)

抗原

See all IL-7 (IL7) ELISA试剂盒
IL-7 (IL7) (Interleukin 7 (IL7))

适用

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检测方法

Colorimetric

实验类型

Sandwich ELISA

检测范围

15.6 pg/mL - 1000 pg/mL

应用范围

ELISA

样品类型

Cell Culture Supernatant, Plasma (EDTA), Serum
  • 最低检测浓度

    15.6 pg/mL

    Supplier Product No.

    koa0459

    Supplier

    Rockland

    原理

    Human IL-7 Sandwich ELISA Kit for Quantitative Detection

    品牌

    AccuSignal™

    Analytical Method

    Quantitative

    特异性

    Production: Natural and recombinant human IL-7. There is no detectable cross-reactivity with other relevant proteins.

    灵敏度

    < 1 pg/mL

    组件

    • Antibody-coated 96-well plate
    • Target Protein Standard
    • Detection antibody
    • Detection reagent
    • Diluent buffers
    • Wash buffers
    • Substrate Solution
    • Stop solutions
    • Adhesive covers
  • 应用备注

    Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL human IL-7 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(EDTA) to each empty well. It is recommended that each human IL-7 standard solution and each sample be measured in duplicate.

    说明

    Standard: Expression system for standard: E.coli, Immunogen sequence: D26-H177

    样本量

    100 μL

    板类型

    Pre-coated

    限制

    仅限研究用
  • 储存条件

    4 °C,-20 °C

    储存方法

    Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

    有效期

    12 months
  • 抗原 See all IL-7 (IL7) ELISA试剂盒

    IL-7 (IL7) (Interleukin 7 (IL7))

    别名

    IL-7

    背景

    Synonyms: IL 7, IL7 nirs variant 6, Interleukin 7, Interleukin7

    Background: IL-7 a hematopoietic growth factor secreted by stromal cells in the red marrow and thymus. It is also produced by keratinocytes,1 dendritic cells,2 hepatocytes,3 neurons, and endothelial cells4. Interleukin 7(IL7) is a protein5 that in humans is encoded by the IL7 gene.6, 7, 8Knockout studies in mice suggested that this cytokine plays an essential role in lymphoid cell survival.9 IL-7 is a cytokine important for B and T cell development. This cytokine and the hepatocyte growth factor(HGF) form a heterodimer that functions as a pre-pro-B cell growth-stimulating factor. This cytokine is found to be a cofactor for V(D)J rearrangement of the T cell receptor beta(TCRB) during early T cell development.

    基因ID

    3574

    NCBI登录号

    NP_000871

    UniProt

    A8K673

    途径

    JAK/STAT Signaling
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