P-Selectin ELISA 试剂盒
Quick Overview for P-Selectin ELISA 试剂盒 (ABIN6719935)
抗原
See all P-Selectin (SELP) ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 62.5 pg/mL
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Supplier Product No.
- koa0302
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Supplier
- Rockland
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原理
- Mouse P-Selectin Sandwich ELISA Kit for Quantitative Detection
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品牌
- AccuSignal™
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Analytical Method
- Quantitative
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特异性
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Production: Natural and recombinant mouse sP-Selectin. There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- < 5 pg/mL
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组件
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- Antibody-coated 96-well plate
- Target Protein Standard
- Detection antibody
- Detection reagent
- Diluent buffers
- Wash buffers
- Substrate Solution
- Stop solutions
- Adhesive covers
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Selectin P (Granule Membrane Protein 140kDa, Antigen CD62) (SELP) ELISA Kit
SELP 适用: 人 Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Selectin P (Granule Membrane Protein 140kDa, Antigen CD62) (SELP) ELISA KitSELP 适用: 小鼠 Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Selectin P (Granule Membrane Protein 140kDa, Antigen CD62) (SELP) ELISA KitSELP 适用: 大鼠 Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Selectin P (Granule Membrane Protein 140kDa, Antigen CD62) (SELP) ELISA KitSELP 适用: 兔 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
Selectin P (Granule Membrane Protein 140kDa, Antigen CD62) (SELP) ELISA KitSELP 适用: Pig Colorimetric Sandwich ELISA 6.25 ng/mL - 400 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Selectin P (Granule Membrane Protein 140kDa, Antigen CD62) (SELP) ELISA KitSELP 适用: 犬 Colorimetric Sandwich ELISA 12.5 ng/mL - 800 ng/mL Plasma, Serum, Tissue Homogenate
Selectin P (Granule Membrane Protein 140kDa, Antigen CD62) (SELP) ELISA KitSELP 适用: 兔 Colorimetric Competition ELISA 31.25 pg/mL - 2000 pg/mL Cell Culture Supernatant, Plasma, Saliva, Serum, Tissue Homogenate, Urine
Selectin P (Granule Membrane Protein 140kDa, Antigen CD62) (SELP) ELISA KitSELP 适用: 人 AA 42-771 Colorimetric Sandwich ELISA 62.5-4000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum
Selectin P (Granule Membrane Protein 140kDa, Antigen CD62) (SELP) ELISA KitSELP 适用: 小鼠 AA 42-709 Colorimetric Sandwich ELISA 62.5-4000 pg/mL Cell Culture Supernatant, Plasma (citrate), Serum
Selectin P (Granule Membrane Protein 140kDa, Antigen CD62) (SELP) ELISA KitSELP 适用: 人 Soluble Colorimetric Sandwich ELISA 78.13-5000 pg/mL Cell Culture Supernatant, Plasma, Serum
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应用备注
- Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL mouse P-Selectin standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(citrate) to each empty well. It is recommended that each mouse P-Selectin standard solution and each sample be measured in duplicate.
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说明
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Standard: Expression system for standard: NSO, Immunogen sequence: W42-A709
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样本量
- 100 μL
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板类型
- Pre-coated
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限制
- 仅限研究用
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
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有效期
- 12 months
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- P-Selectin (SELP) (Selectin P (Granule Membrane Protein 140kDa, Antigen CD62) (SELP))
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别名
- P-Selectin
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背景
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Synonyms: Antigen CD62, CD62, CD62 antigen like family member P, GMP140, Granule membrane protein 140, Granule membrane protein 140 kDa, GRMP, LECAM3, Leukocyte endothelial cell adhesion molecule 3, Leukocyte-endothelial cell adhesion molecule 3, LYAM3_HUMAN, P selectin, P-selectin, PADGEM, Platelet activation dependent granule external membrane protein, Platelet activation dependent granule-external membrane protein, Platelet alpha granule membrane protein, PSEL, Selectin P
Background: P-selectin, also called GMP-140, CD62, or selectin P, is a 140-kD adhesion molecule, expressed at the surface of activated cells, that mediates the interaction of activated endothelial cells or platelets with leukocytes. It is stored in secretory granules and expressed at the plasma membrane after cell activation. It is known to play an important role in atherosclerosis. The major ligand for P-selectin on leukocytes is P-selectin glycoprotein ligand-1(PSGL-1). The standard product used in this kit is recombinant mouse P-Selectin, excluding intercellular P-Selectin and transmembrane domain. It has 42-709 amino acids sequence with the molecular mass of 99.1KDa. As a result of glycosylation, the molecular mass of 190-200KDa is revealed by SDS-PAGE.
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基因ID
- 20344
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NCBI登录号
- NP_035477
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UniProt
- Q01102
抗原 See all P-Selectin (SELP) ELISA试剂盒
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