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alpha Fetoprotein ELISA 试剂盒

AFP 适用: 人 Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
产品编号 ABIN6719930
发货至: 中国
  • 抗原 See all alpha Fetoprotein (AFP) ELISA试剂盒
    alpha Fetoprotein (AFP) (alpha-Fetoprotein (AFP))
    适用
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    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    检测范围
    78 pg/mL - 5000 pg/mL
    最低检测浓度
    78 pg/mL
    应用范围
    ELISA
    原理
    Sandwich ELISA for Quantitative Detection of Antigen
    样品类型
    Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
    Analytical Method
    Quantitative
    产品特性

    Synonyms: Afp, Alpha fetoglobulin, Alpha-fetoglobulin, Alpha fetoprotein, Alpha-fetoprotein, Alpha-1-fetoprotein, FETA, HPAFP

    Background: Alpha-fetoprotein (AFP, α-fetoprotein, also sometimes called alpha-1-fetoprotein, alpha-fetoglobulin, or alpha fetal protein) is a protein that in humans is encoded by the AFP gene. The AFP gene is located on the q arm of chromosome 4 (4q25). AFP is the most abundant plasma protein found in the human fetus. Plasma levels decrease rapidly after birth but begin decreasing prenatally starting at the end of the first trimester. Normal adult levels are usually achieved by the age of 8 to 12 months. The function of AFP in adult humans is unknown, however, in rodents it binds estradiol to prevent the transport of this hormone across the placenta to the fetus. The main function of this is to prevent the virilization of female fetuses. As human AFP does not bind estrogen, its function in humans is less clear.

    Gene Name: AFP

    Production: Natural and recombinant human AFP. There is no detectable cross-reactivity with other relevant proteins.

    Standard: Expression system for standard: NSO, Immunogen sequence: R19-V609

    Top Product
    Discover our top product AFP ELISA Kit
  • 应用备注

    Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 313pg/mL, 156pg/mL, 78pg/mL human AFP standard solutions into the pre-coated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma( heparin, EDTA) to each empty well. We recommend that each human AFP standard solution and each sample is measured in duplicate.

    Blood Product Anticoagulant: Heparin Sodium

    ELISA Dilution: 78pg/mL-5000pg/mL

    样本量
    100 μL
    板类型
    Pre-coated
    限制
    仅限研究用
  • 储存条件
    RT,4 °C,-20 °C
    储存方法
    Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
  • 抗原 See all alpha Fetoprotein (AFP) ELISA试剂盒
    alpha Fetoprotein (AFP) (alpha-Fetoprotein (AFP))
    别名
    AFP (AFP 产品)
    别名
    FETA ELISA Kit, HPAFP ELISA Kit, alpha fetoprotein ELISA Kit, alpha-fetoprotein ELISA Kit, AFP ELISA Kit, Afp ELISA Kit
    基因ID
    101
    NCBI登录号
    NP_001100
    UniProt
    P78325
    途径
    C21-Steroid Hormone Metabolic Process
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