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Fetuin A ELISA 试剂盒

This 人 Fetuin A ELISA Kit is a Colorimetric ELISA Kit designed to quantify 人 Fetuin A.
Rockland
产品编号 ABIN6719928
Supplier Product No.: koa0448
发货至: 中国

Quick Overview for Fetuin A ELISA 试剂盒 (ABIN6719928)

抗原

See all Fetuin A (AHSG) ELISA试剂盒
Fetuin A (AHSG) (alpha-2-HS-Glycoprotein (AHSG))

适用

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检测方法

Colorimetric

实验类型

Sandwich ELISA

检测范围

312 pg/mL - 20000 pg/mL

应用范围

ELISA

样品类型

Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
  • 最低检测浓度

    312 pg/mL

    Supplier Product No.

    koa0448

    Supplier

    Rockland

    原理

    Human Fetuin A Sandwich ELISA Kit for Quantitative Detection

    品牌

    AccuSignal™

    Analytical Method

    Quantitative

    特异性

    Production: Natural and recombinant human Fetuin A. There is no detectable cross-reactivity with other relevant proteins.

    灵敏度

    < 10 pg/mL

    组件

    • Antibody-coated 96-well plate
    • Target Protein Standard
    • Detection antibody
    • Detection reagent
    • Diluent buffers
    • Wash buffers
    • Substrate Solution
    • Stop solutions
    • Adhesive covers
  • 应用备注

    Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 20000pg/mL, 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL human Fetuin A standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma( heparin, EDTA) to each empty well. It is recommended that each human Fetuin A standard solution and each sample be measured in duplicate.

    说明

    Standard: Expression system for standard: NSO, Immunogen sequence: A19-V367

    样本量

    100 μL

    板类型

    Pre-coated

    限制

    仅限研究用
  • 储存条件

    4 °C,-20 °C

    储存方法

    Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

    有效期

    12 months
  • 抗原 See all Fetuin A (AHSG) ELISA试剂盒

    Fetuin A (AHSG) (alpha-2-HS-Glycoprotein (AHSG))

    别名

    Fetuin A

    背景

    Synonyms: A2HS, AHS, Ahsg, Alpha 2 HS glycoprotein, Alpha-2-HS-glycoprotein chain B, Alpha-2-Z-globulin, Ba-alpha-2-glycoprotein, FETUA, FETUA_HUMAN, Fetuin-A, HSGA

    Background: Alpha2-HS glycoprotein(AHSG), also known as fetuin-A, is a plasma protein displaying high-affinity interaction with calcium phosphate, by which ectopic vascular calcification is prevented. The AHSG polymorphism is attributable to the hereditary variation of AHSG and phosphate serum levels, which may affect skeletal development and chronic disorders such as vascular calcification.1Human plasma protein alpha 2-HS-glycoprotein(AHSG) is composed of two polypeptide chains, A and B, encoded by a single mRNA. Southern blot analysis of mouse x human somatic cell hybrids has mapped the AHSG gene to human chromosome 3 in the region 3q21----qter. Using a recombinant plasmid containing a 1,538 bp insert spanning the entire AHSG coding region, AHSG was localized to chromosomal bands 3q27----q29 by in situ hybridization.2

    基因ID

    197

    NCBI登录号

    NP_001613

    UniProt

    P02765
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