CD13 ELISA 试剂盒
Quick Overview for CD13 ELISA 试剂盒 (ABIN6719920)
抗原
See all CD13 (ANPEP) ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 312 pg/mL
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Supplier Product No.
- koa0715
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Supplier
- Rockland
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原理
- Mouse CD13 - Aminopeptidase N Sandwich ELISA Kit for Quantitative Detection
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品牌
- AccuSignal™
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Analytical Method
- Quantitative
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特异性
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Production: Natural and recombinant mouse CD13. There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- < 10 pg/mL
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组件
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- Antibody-coated 96-well plate
- Target Protein Standard
- Detection antibody
- Detection reagent
- Diluent buffers
- Wash buffers
- Substrate Solution
- Stop solutions
- Adhesive covers
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Alanyl (Membrane) Aminopeptidase (ANPEP) ELISA Kit
ANPEP 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate, Urine
Alanyl (Membrane) Aminopeptidase (ANPEP) ELISA KitANPEP 适用: 大鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Alanyl (Membrane) Aminopeptidase (ANPEP) ELISA KitANPEP 适用: 小鼠 Colorimetric Sandwich ELISA 0.781 ng/mL - 50 ng/mL Plasma, Serum, Tissue Homogenate
Alanyl (Membrane) Aminopeptidase (ANPEP) ELISA KitANPEP 适用: 人 AA 69-967 Colorimetric Sandwich ELISA 312-20.000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Alanyl (Membrane) Aminopeptidase (ANPEP) ELISA KitANPEP 适用: 小鼠 AA 69-966 Colorimetric Sandwich ELISA 312-20.000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
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应用备注
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ELISA_Dilution: 312pg/mL-20,000pg/mL
Application_Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 20,000pg/mL, 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL mouse CD13 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each mouse CD13 standard solution and each sample be measured in duplicate.
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说明
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Standard: Expression system for standard: NSO, Immunogen sequence: K69-S966
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样本量
- 100 μL
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板类型
- Pre-coated
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限制
- 仅限研究用
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
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有效期
- 12 months
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- CD13 (ANPEP) (Alanyl (Membrane) Aminopeptidase (ANPEP))
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别名
- CD13/Aminopeptidase N
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背景
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Synonyms: Alanyl (membrane) aminopeptidase, Alanyl aminopeptidase, Alanyl membrane aminopeptidase, Aminopeptidase M, Aminopeptidase N, AMPN_HUMAN, ANPEP, AP M, AP N, AP-M, AP-N, APN, CD 13, CD13, CD13 antigen, gp150, hAPN, Lap 1, Lap1, Microsomal aminopeptidase, Myeloid plasma membrane glycoprotein CD13, p150, PEPN
Background: Alanine aminopeptidase, also known as ANPEP or CD13. is an enzyme that is used as a biomarker to detect damage to the kidneys, and that may be used to help diagnose certain kidney disorders. It is mapped to 15q26.1. Aminopeptidase N is located in the small-intestinal and renal microvillar membrane, and also in other plasma membranes. In the small intestine, Aminopeptidase N plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases, and it is also thought to be involved in the metabolism of regulatory peptides by diverse cell types, including small intestinal and renal tubular epithelial cells, macrophages, granulocytes, and synaptic membranes from the CNS. Petrovic et al showed that CD13 was required for endothelial cell invasion in response to bradykinin. Inhibition of CD13 abrogated internalization of bradykinin receptor B2 and reduced endothelial cell motility.
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基因ID
- 16790
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NCBI登录号
- NP_032512
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UniProt
- P97449
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途径
- Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones
抗原 See all CD13 (ANPEP) ELISA试剂盒
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