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Galectin 2 ELISA 试剂盒

This 人 Galectin 2 ELISA Kit is a Colorimetric ELISA Kit designed to quantify 人 Galectin 2 in Cell Culture Lysate, Cell Culture Supernatant, Plasma (EDTA - heparin), Serum 和 Tissue Homogenate.
Rockland
产品编号 ABIN6719894
Supplier Product No.: koa0754
发货至: 中国

Quick Overview for Galectin 2 ELISA 试剂盒 (ABIN6719894)

抗原

See all Galectin 2 (LGALS2) ELISA试剂盒
Galectin 2 (LGALS2) (Lectin, Galactoside-Binding, Soluble, 2 (LGALS2))

适用

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检测方法

Colorimetric

实验类型

Sandwich ELISA

检测范围

156 pg/mL - 10000 pg/mL

应用范围

ELISA

样品类型

Cell Culture Lysate, Cell Culture Supernatant, Plasma (EDTA - heparin), Serum, Tissue Homogenate
  • 最低检测浓度

    156 pg/mL

    Supplier Product No.

    koa0754

    Supplier

    Rockland

    原理

    Human Galectin-2 Sandwich ELISA Kit for Quantitative Detection

    品牌

    AccuSignal™

    Analytical Method

    Quantitative

    特异性

    Production: Natural and recombinant human Galectin-2. There is no detectable cross-reactivity with other relevant proteins.

    灵敏度

    < 10 pg/mL

    组件

    • Antibody-coated 96-well plate
    • Target Protein Standard
    • Detection antibody
    • Detection reagent
    • Diluent buffers
    • Wash buffers
    • Substrate Solution
    • Stop solutions
    • Adhesive covers
  • 应用备注

    ELISA_Dilution: 156pg/mL-10,000pg/mL

    Application_Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human Galectin-2 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human Galectin-2 standard solution and each sample be measured in duplicate.

    说明

    Standard: Expression system for standard: E.coli, Immunogen sequence: M1-E132

    样本量

    100 μL

    板类型

    Pre-coated

    限制

    仅限研究用
  • 储存条件

    4 °C,-20 °C

    储存方法

    Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

    有效期

    12 months
  • 抗原 See all Galectin 2 (LGALS2) ELISA试剂盒

    Galectin 2 (LGALS2) (Lectin, Galactoside-Binding, Soluble, 2 (LGALS2))

    别名

    Galectin-2

    背景

    Synonyms: Beta galactoside binding lectin L14 II, Beta-galactoside-binding lectin L-14-II, CBP35, Gal-2, GAL3, GALBP, Galectin 2, Galectin-2, GALIG, HL14, Lactose binding lectin 2, Lactose-binding lectin 2, LEG2_HUMAN, Lgals2, MAC2, S Lac lectin 2, S-Lac lectin 2

    Background: Galectin-2 is a protein that in humans is encoded by the LGALS2 gene. It is mapped to 22q13.1. The protein encoded by this gene is a soluble beta-galactoside binding lectin. The encoded protein is found as a homodimer and can bind to lymphotoxin-alpha. It has been found that a single-nucleotide polymorphism in Galectin-2 is significantly associated with susceptibility to myocardial infarction. This genetic substitution affects the transcriptional level of Galectin-2 in vitro, potentially leading to altered secretion of LTA, to which Galectin-2 binds, which would then affect the degree of inflammation.

    基因ID

    3957

    NCBI登录号

    NP_006489

    UniProt

    P05162
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