Myeloperoxidase ELISA 试剂盒
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Quick Overview for Myeloperoxidase ELISA 试剂盒 (ABIN6719875)
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See all Myeloperoxidase (MPO) ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 312 pg/mL
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Supplier Product No.
- koa0511
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Supplier
- Rockland
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原理
- Human MPO Sandwich ELISA Kit for Quantitative Detection
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品牌
- AccuSignal™
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Analytical Method
- Quantitative
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特异性
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Expression system for standard: NS0
Immunogen sequence: A49-S745 -
交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- < 10 pg/mL
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组件
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- Antibody-coated 96-well plate
- Target Protein Standard
- Detection antibody
- Detection reagent
- Diluent buffers
- Wash buffers
- Substrate Solution
- Stop solutions
- Adhesive covers
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试剂未包括
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.
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Myeloperoxidase (MPO) ELISA Kit
MPO 适用: 小鼠 Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Myeloperoxidase (MPO) ELISA KitMPO 适用: 大鼠 Colorimetric Sandwich ELISA 3.12 ng/mL - 200 ng/mL Plasma, Serum, Tissue Homogenate
Myeloperoxidase (MPO) ELISA KitMPO 适用: 小鼠 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Myeloperoxidase (MPO) ELISA KitMPO 适用: Pig Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Myeloperoxidase (MPO) ELISA KitMPO 适用: 人 Colorimetric Sandwich ELISA 7.8 ng/mL - 500 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Myeloperoxidase (MPO) ELISA KitMPO 适用: Cow Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Myeloperoxidase (MPO) ELISA KitMPO 适用: 大鼠 Colorimetric Sandwich ELISA 3.12 ng/mL - 200 ng/mL Plasma, Serum, Tissue Homogenate
Myeloperoxidase (MPO) ELISA KitMPO 适用: 犬 Colorimetric Sandwich ELISA 31.25 ng/mL - 2000 ng/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
Myeloperoxidase (MPO) ELISA KitMPO 适用: Pig Colorimetric Sandwich ELISA 1.563 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
Myeloperoxidase (MPO) ELISA KitMPO 适用: Cow Colorimetric Sandwich ELISA 1.563 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
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说明
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Capture antibody and Detection antibody: monoclonal antibody from mouse, polyclonal antibody from goat
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样本量
- 100 μL
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板类型
- Pre-coated
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实验流程
- Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 20000pg/mL, 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL human MPO standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, cell lysate, tissue lysate, serum, plasma(heparin, EDTA), saliva or urine to each empty well. It is recommended that each human MPO standard solution and each sample be measured in duplicate.
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实验精密度
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Intra-Assay Precision (CV%): 7.4%, 6.8%, 4.2%
Inter-Assay Precision (CV%): 8.2%, 7.9%, 5.5% -
限制
- 仅限研究用
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
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有效期
- 12 months
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- Myeloperoxidase (MPO)
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别名
- MPO
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背景
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Background: Myeloperoxidase (MPO) is a mammalian phagocyte hemoprotein thought to primarily mediate host defense reactions. It is abundantly expressed in neutrophils and secreted during their activation. Myeloperoxidase is part of the host defense system of human polymorphonuclear leukocytes, responsible for microbicidal activity against a wide range of organisms. It is located in the nucleus as well as in the cytoplasm. Intranuclear MPO may help to protect DNA against damage resulting from oxygen radicals produced during myeloid cell maturation and function. The standard product used in this kit is the product of gene recombination, consisting of 697 (A49-S745) amino acids with the molecular mass of 80KDa.
Gene Full Name: myeloperoxidase
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基因ID
- 4353
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NCBI登录号
- NP_000241
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UniProt
- P05164
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途径
- Chromatin Binding
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