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BMP2 ELISA 试剂盒

This 大鼠 BMP2 ELISA Kit is a Colorimetric ELISA Kit designed to quantify 大鼠 BMP2.
Rockland
产品编号 ABIN6719858
Supplier Product No.: koa0151
发货至: 中国

Quick Overview for BMP2 ELISA 试剂盒 (ABIN6719858)

抗原

See all BMP2 ELISA试剂盒
BMP2 (Bone Morphogenetic Protein 2 (BMP2))

适用

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大鼠

检测方法

Colorimetric

实验类型

Sandwich ELISA

检测范围

62.5 pg/mL - 4000 pg/mL

应用范围

ELISA

样品类型

Cell Culture Supernatant, Serum
  • 最低检测浓度

    62.5 pg/mL

    Supplier Product No.

    koa0151

    Supplier

    Rockland

    原理

    Rat BMP-2 Sandwich ELISA Kit for Quantitative Detection

    品牌

    AccuSignal™

    Analytical Method

    Quantitative

    特异性

    Production: Natural and recombinant rat BMP-2. There is no detectable cross-reactivity with other relevant proteins.

    灵敏度

    < 2 pg/mL

    组件

    • Antibody-coated 96-well plate
    • Target Protein Standard
    • Detection antibody
    • Detection reagent
    • Diluent buffers
    • Wash buffers
    • Substrate Solution
    • Stop solutions
    • Adhesive covers
  • 应用备注

    Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL rat BMP-2 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of rat bone tissue, cell culture supernates or serum to each empty well. It is recommended that each rat BMP-2 standard solution and each sample be measured in duplicate.

    说明

    Standard: Expression system for standard:CHO , Immunogen sequence: Q283-R396

    样本量

    100 μL

    板类型

    Pre-coated

    限制

    仅限研究用
  • 储存条件

    4 °C,-20 °C

    储存方法

    Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

    有效期

    12 months
  • 抗原 See all BMP2 ELISA试剂盒

    BMP2 (Bone Morphogenetic Protein 2 (BMP2))

    别名

    BMP-2

    背景

    Synonyms: BDA2, BMP-2, BMP-2A, Bmp2, BMP2_HUMAN, BMP2A, Bone morphogenetic protein 2, Bone morphogenetic protein 2A

    Background: Bone morphogenetic protein-2(BMP-2, BMP 2A) belongs to the transforming growth factor beta(TGF-beta) superfamily. It is thought to be involved in cartilage and bone formation during embryogenesis, but may have additional functions in morphogenesis as implied by its expression in various organs and embryonic tissues of mice. BMP-2 has been identified as a candidate mediator of retinoid activity. BMP-2 protein induces medulloblastoma cell apoptosis, whereas the BMP-2 antagonist noggin blocks both retinoid and BMP-2-induced apoptosis. BMP-2 also induces p38 mitogen-activated protein kinase(MAPK), which is necessary for BMP-2 and retinoid-induced apoptosis. Bone morphogenic proteins(BMPs) are known to promote osteogenesis, and clinical trials are currently underway to evaluate the ability of certain BMPs to promote fracture-healing and spinal fusion. The standard product used in this kit is recombinant rat BMP-2, constituting dimer by two chains of 114 amino acids with the molecular mass of 26KDa.

    基因ID

    29373

    NCBI登录号

    NP_058874

    UniProt

    P49001

    途径

    Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Regulation of Muscle Cell Differentiation, Growth Factor Binding, Positive Regulation of fat Cell Differentiation
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