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TFPI2 ELISA 试剂盒

This 人 TFPI2 ELISA Kit is a Colorimetric ELISA Kit designed to quantify 人 TFPI2.
Rockland
产品编号 ABIN6719845
Supplier Product No.: koa0821
发货至: 中国

Quick Overview for TFPI2 ELISA 试剂盒 (ABIN6719845)

抗原

See all TFPI2 ELISA试剂盒
TFPI2 (Tissue Factor Pathway Inhibitor 2 (TFPI2))

适用

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检测方法

Colorimetric

实验类型

Sandwich ELISA

检测范围

156 pg/mL - 10000 pg/mL

应用范围

ELISA

样品类型

Cell Culture Supernatant, Serum
  • 最低检测浓度

    156 pg/mL

    Supplier Product No.

    koa0821

    Supplier

    Rockland

    原理

    Human TFPI2 Sandwich ELISA Kit for Quantitative Detection

    品牌

    AccuSignal™

    Analytical Method

    Quantitative

    特异性

    Production: Natural and recombinant human TFPI2. There is no detectable cross-reactivity with other relevant proteins.

    灵敏度

    < 10 pg/mL

    组件

    • Antibody-coated 96-well plate
    • Target Protein Standard
    • Detection antibody
    • Detection reagent
    • Diluent buffers
    • Wash buffers
    • Substrate Solution
    • Stop solutions
    • Adhesive covers
  • 应用备注

    Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human TFPI2 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates or serum to each empty well. It is recommended that each human TFPI2 standard solution and each sample be measured in duplicate.

    说明

    Standard: Expression system for standard: NSO, Immunogen sequence: D23-F235

    样本量

    100 μL

    板类型

    Pre-coated

    限制

    仅限研究用
  • 储存条件

    4 °C,-20 °C

    储存方法

    Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

    有效期

    12 months
  • 抗原 See all TFPI2 ELISA试剂盒

    TFPI2 (Tissue Factor Pathway Inhibitor 2 (TFPI2))

    别名

    TFPI2

    背景

    Synonyms: Placental protein 5, PP5, REF1, Retinal pigment epithelium cell factor 1, TFPI 2, TFPI-2, TFPI2, TFPI2 PEN, TFPI2_HUMAN, Tissue factor pathway inhibitor 2

    Background: TFPI2, also called Tissue factor pathway inhibitor 2, is a protein that in humans is encoded by the TFPI2 gene. It is mapped to 7q21.3. This gene encodes a member of the Kunitz-type serine proteinase inhibitor family. The protein can inhibit a variety of serine proteases including factor VIIa/tissue factor, factor Xa, plasmin, trypsin, chymotryspin and plasma kallikrein. This gene has been identified as a tumor suppressor gene in several types of cancer. Qualitative PCR and microarray analyses showed that expression of TFPI2 was inversely correlated with that of MIR616 in prostate cancer cell lines, primary tumors of various grades, and normal tissue.

    基因ID

    7980

    NCBI登录号

    NP_001257932

    UniProt

    P48307
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