EPO ELISA 试剂盒
Quick Overview for EPO ELISA 试剂盒 (ABIN6719830)
抗原
See all EPO ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 46.9 pg/mL
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Supplier Product No.
- koa0162
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Supplier
- Rockland
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原理
- Mouse EPO Sandwich ELISA Kit for Quantitative Detection
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品牌
- AccuSignal™
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Analytical Method
- Quantitative
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特异性
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Production: Natural and recombinant mouse EPO. There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- < 15 pg/mL
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组件
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- Antibody-coated 96-well plate
- Target Protein Standard
- Detection antibody
- Detection reagent
- Diluent buffers
- Wash buffers
- Substrate Solution
- Stop solutions
- Adhesive covers
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Erythropoietin (EPO) ELISA Kit
EPO 适用: 人 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Erythropoietin (EPO) ELISA KitEPO 适用: 大鼠 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Cell Culture Supernatant, Plasma, Serum
Erythropoietin (EPO) ELISA KitEPO 适用: 大鼠 Colorimetric Sandwich ELISA 93.75 pg/mL - 6000 pg/mL Plasma, Serum, Tissue Homogenate
Erythropoietin (EPO) ELISA KitEPO 适用: 犬 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Plasma, Serum
Erythropoietin (EPO) ELISA KitEPO 适用: 马 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Erythropoietin (EPO) ELISA KitEPO 适用: Pig Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Plasma, Serum
Erythropoietin (EPO) ELISA KitEPO 适用: 小鼠 Colorimetric Sandwich ELISA 78.13 pg/mL - 5000 pg/mL Cell Culture Supernatant, Plasma, Serum
Erythropoietin (EPO) ELISA KitEPO 适用: 猴 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Erythropoietin (EPO) ELISA KitEPO 适用: 小鼠 Colorimetric Sandwich ELISA 6.25 pg/mL - 400 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Erythropoietin (EPO) ELISA KitEPO 适用: 猴 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Cell Culture Supernatant, Plasma, Serum
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应用备注
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ELISA_Dilution: 46.9pg/mL-3000pg/mL
Application_Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 3000pg/mL, 1500pg/mL, 750pg/mL,419.pg/mL, 187.5pg/mL, 93.8pg/mL, 46.9pg/mL mouse EPO standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernatants, serum, plasma(heparin) or tissue lysates to each empty well. We recommend that each mouse EPO standard solution and each sample is measured in duplicate.
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说明
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Standard: Expression system for standard: NSO, Immunogen sequence: A27-R192
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样本量
- 100 μL
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板类型
- Pre-coated
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限制
- 仅限研究用
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
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有效期
- 12 months
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- EPO (Erythropoietin (EPO))
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别名
- EPO
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物质类
- Hormone
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背景
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Synonyms: C C motif chemokine 24, CCL24, Chemokine (C C motif) ligand 24, Chemokine CC Motif Ligand 2, CK beta 6, Ckb 6, Ckb6, Eosinophil chemotactic protein 2, Eotaxin 2 like protein, MPIF 2, MPIF2, Myeloid progenitor inhibitory factor 2, OTTHUMP00000210225, SCYA24, Small inducible cytokine A24, Small inducible cytokine subfamily A (Cys Cys) member 24
Background: Erythropoietin, EPO, also known as hematopoietin or hemopoietin, is a glycoprotein hormone that controls erythropoiesis, or red blood cell production. It is a cytokine for erythrocyte(red blood cell) precursors in the bone marrow. Its gene is mapped to 7q22. It is said that the EPO gene encodes a deduced 193-amino acid propolypeptide. This hormone can be found in kidney and liver. It is the hormone that regulates red blood cell production. And it plays an important role in the brain's response to neuronal injury. What's more, EPO is also involved in the wound healing process.
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基因ID
- 13856
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NCBI登录号
- NP_031968
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UniProt
- P07321
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途径
- JAK/STAT Signaling, Hormone Activity, Negative Regulation of intrinsic apoptotic Signaling, Negative Regulation of Transporter Activity
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