CD32-B/C ELISA 试剂盒
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北京 101111
Quick Overview for CD32-B/C ELISA 试剂盒 (ABIN6719821)
抗原
适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 46.9 pg/mL
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Supplier Product No.
- koa0785
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Supplier
- Rockland
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原理
- Human CD32 - FCGR2b - c Sandwich ELISA Kit for Quantitative Detection
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品牌
- AccuSignal™
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Analytical Method
- Quantitative
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特异性
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Expression system for standard: NS0
Immunogen sequence: A46-P217 -
交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- < 10 pg/mL
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组件
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- Antibody-coated 96-well plate
- Target Protein Standard
- Detection antibody
- Detection reagent
- Diluent buffers
- Wash buffers
- Substrate Solution
- Stop solutions
- Adhesive covers
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试剂未包括
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.
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CD32-B/C ELISA Kit
适用: 人 Colorimetric Sandwich ELISA 85 pg/mL - 5000 pg/mL Cell Culture Supernatant, Plasma, Serum
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说明
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Capture antibody and Detection antibody: monoclonal antibody from mouse, polyclonal antibody from goat
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样本量
- 100 μL
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板类型
- Pre-coated
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实验流程
- Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 3000pg/mL, 1500pg/mL, 750pg/mL, 419.pg/mL, 187.5pg/mL, 93.8pg/mL, 46.9pg/mL human CD32 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernatants, serum or plasma(heparin, EDTA) to each empty well. We recommend that each human CD32 standard solution and each sample is measured in duplicate.
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实验精密度
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Intra-Assay Precision (CV%): 6.6%, 4.9%, 7.1%
Inter-Assay Precision (CV%): 7.3%, 6.1%, 8.8% -
限制
- 仅限研究用
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
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有效期
- 12 months
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- CD32-B/C
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别名
- CD32/FCGR2b/c
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背景
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Background: CD32 is a surface receptor protein and part of a large population of B cell co-receptors, which act to modulate signaling. It has a low-affinity for IgG antibodies and down-regulates antibody production in the presence of IgG. This feedback loops essentially lowers the production of IgG produced by B cells when there is a surplus in the body.
Gene Full Name: Fc fragment of IgG, low affinity IIb, receptor (CD32)
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基因ID
- 2213
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NCBI登录号
- NP_963857
抗原
-
