LGALS3BP ELISA 试剂盒
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Quick Overview for LGALS3BP ELISA 试剂盒 (ABIN6719782)
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See all LGALS3BP ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 156 pg/mL
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Supplier Product No.
- koa0729
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Supplier
- Rockland
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原理
- Human Galectin-3BP Sandwich ELISA Kit for Quantitative Detection
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品牌
- AccuSignal™
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Analytical Method
- Quantitative
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特异性
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Expression system for standard: NS0
Immunogen sequence: V19-D585 -
交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- < 10 pg/mL
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组件
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- Antibody-coated 96-well plate
- Target Protein Standard
- Detection antibody
- Detection reagent
- Diluent buffers
- Wash buffers
- Substrate Solution
- Stop solutions
- Adhesive covers
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试剂未包括
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.
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Lectin, Galactoside-Binding, Soluble, 3 Binding Protein (LGALS3BP) ELISA Kit
LGALS3BP 适用: 人 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Lectin, Galactoside-Binding, Soluble, 3 Binding Protein (LGALS3BP) ELISA KitLGALS3BP 适用: 人 Colorimetric Sandwich ELISA 1.25 ng/mL - 80 ng/mL Plasma, Serum, Tissue Homogenate
Lectin, Galactoside-Binding, Soluble, 3 Binding Protein (LGALS3BP) ELISA KitLGALS3BP 适用: 大鼠 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
Lectin, Galactoside-Binding, Soluble, 3 Binding Protein (LGALS3BP) ELISA KitLGALS3BP 适用: 小鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum
Lectin, Galactoside-Binding, Soluble, 3 Binding Protein (LGALS3BP) ELISA KitLGALS3BP 适用: 小鼠 Colorimetric Sandwich ELISA 0.39 ng/mL - 25 ng/mL Plasma, Serum, Tissue Homogenate
Lectin, Galactoside-Binding, Soluble, 3 Binding Protein (LGALS3BP) ELISA KitLGALS3BP 适用: 人 Colorimetric Sandwich ELISA 1.56-100 ng/mL Plasma, Serum, Tissue Homogenate
Lectin, Galactoside-Binding, Soluble, 3 Binding Protein (LGALS3BP) ELISA KitLGALS3BP 适用: 人 AA 19-585 Colorimetric Sandwich ELISA 156 pg/mL - 10000 pg/mL Cell Culture Supernatant, Plasma (heparin), Serum
Lectin, Galactoside-Binding, Soluble, 3 Binding Protein (LGALS3BP) ELISA KitLGALS3BP 适用: 人 Colorimetric Sandwich ELISA 1.25 ng/mL - 80 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Lectin, Galactoside-Binding, Soluble, 3 Binding Protein (LGALS3BP) ELISA KitLGALS3BP 适用: 人 Colorimetric Sandwich ELISA 1.25 ng/mL - 80 ng/mL Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
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说明
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Capture antibody and Detection antibody: monoclonal antibody from mouse, monoclonal antibody from mouse
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样本量
- 100 μL
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板类型
- Pre-coated
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实验流程
- Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL human Galectin-3BP standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin) to each empty well. It is recommended that each human Galectin-3BP standard solution and each sample be measured in duplicate.
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实验精密度
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Intra-Assay Precision (CV%): 6%, 5.8%, 6.8%
Inter-Assay Precision (CV%): 6.4%, 7.3%, 7.2% -
限制
- 仅限研究用
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
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有效期
- 12 months
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- LGALS3BP (Lectin, Galactoside-Binding, Soluble, 3 Binding Protein (LGALS3BP))
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别名
- Galectin-3BP
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背景
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Background: Galectin-3-binding protein is a protein that in humans is encoded by the LGALS3BP gene. The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. Using fluorescence in-situ hybridization, the full length 90K cDNA has been localized to chromosome 17q25. The native protein binds specifically to a human macrophage-associated lectin known as Mac-2 and also binds to galectin 1.
Gene Full Name: galectin 3 binding protein
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基因ID
- 3959
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NCBI登录号
- NP_005558
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UniProt
- Q08380
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