LGALS7 ELISA 试剂盒
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北京 101111
Quick Overview for LGALS7 ELISA 试剂盒 (ABIN6719778)
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See all LGALS7 ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 93.8 pg/mL
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Supplier Product No.
- koa0755
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Supplier
- Rockland
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原理
- Mouse Galectin-7 Sandwich ELISA Kit for Quantitative Detection
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品牌
- AccuSignal™
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Analytical Method
- Quantitative
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特异性
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Expression system for standard: E.coli
Immunogen sequence: S2-F136 -
交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- < 10 pg/mL
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组件
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- Antibody-coated 96-well plate
- Target Protein Standard
- Detection antibody
- Detection reagent
- Diluent buffers
- Wash buffers
- Substrate Solution
- Stop solutions
- Adhesive covers
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试剂未包括
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.
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Galectin 7 (LGALS7) ELISA Kit
LGALS7 适用: 人 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Galectin 7 (LGALS7) ELISA KitLGALS7 适用: 小鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Galectin 7 (LGALS7) ELISA KitLGALS7 适用: 大鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Galectin 7 (LGALS7) ELISA KitLGALS7 适用: 人 Colorimetric Sandwich ELISA 125 pg/mL - 8000 pg/mL Plasma, Serum, Tissue Homogenate
Galectin 7 (LGALS7) ELISA KitLGALS7 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Galectin 7 (LGALS7) ELISA KitLGALS7 适用: 人 AA 2-136 Colorimetric Sandwich ELISA 156 pg/mL - 10000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma (EDTA), Plasma (heparin), Serum
Galectin 7 (LGALS7) ELISA KitLGALS7 适用: 小鼠 AA 2-136 Colorimetric Sandwich ELISA 93.8 pg/mL - 6000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma (EDTA), Plasma (heparin), Serum
Galectin 7 (LGALS7) ELISA KitLGALS7 适用: 人 Colorimetric 78-5000 pg/mL Plasma, Serum, Tissue Homogenate
Galectin 7 (LGALS7) ELISA KitLGALS7 适用: 小鼠 Colorimetric Sandwich ELISA 65 pg/mL - 16000 pg/mL Cell Culture Supernatant, Plasma, Serum
Galectin 7 (LGALS7) ELISA KitLGALS7 适用: 人 Colorimetric Sandwich ELISA 125 pg/mL - 8000 pg/mL Cell Culture Supernatant, Plasma, Serum
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说明
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Capture antibody and Detection antibody: monoclonal antibody from rat, polyclonal antibody from goat
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样本量
- 100 μL
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板类型
- Pre-coated
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实验流程
- Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 6000pg/mL, 3000pg/mL, 1500pg/mL, 750pg/mL, 419pg/mL, 187.5pg/mL, 93.8pg/mL mouse Galectin-7 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. We recommend that each mouse Galectin-7 standard solution and each sample is measured in duplicate.
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实验精密度
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Intra-Assay Precision (CV%): 4.1%, 4.7%, 7.9%
Inter-Assay Precision (CV%): 4.9%, 5.3%, 8.4% -
限制
- 仅限研究用
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
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有效期
- 12 months
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- LGALS7 (Galectin 7 (LGALS7))
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别名
- Galectin-7
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背景
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Background: Galectin-7, also known as GAL7, is a protein that in humans is encoded by the LGALS7 gene. This gene is mapped to 19q13.2. Galectins belong to a family of related beta-galactoside-binding lectins, also referred to as S-type or S-Lac lectins. Members of this family have been implicated in a variety of functions, including growth regulation, cell adhesion, migration, neoplastic transformation, and immune responses. Differential and in situ hybridizations indicate that this lectin is specifically expressed in keratinocytes. It has been found that expression of Galectin-7 was markedly upregulated in corneal epithelium after injury and that exogenous galectin-7 stimulated reepithelialization of corneal wounds.
Gene Full Name: Galectin-7
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基因ID
- 16858
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NCBI登录号
- NP_032522
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UniProt
- O54974
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