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CD5 ELISA 试剂盒

CD5 适用: 人 Colorimetric Sandwich ELISA 93.7 pg/mL - 6000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
产品编号 ABIN6719736
发货至: 中国
  • 抗原 See all CD5 ELISA试剂盒
    CD5
    适用
    • 5
    • 2
    • 2
    • 1
    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    检测范围
    93.7 pg/mL - 6000 pg/mL
    最低检测浓度
    93.7 pg/mL
    应用范围
    ELISA
    原理
    Sandwich ELISA for Quantitative Detection of Antigen
    样品类型
    Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
    Analytical Method
    Quantitative
    产品特性

    Synonyms: CD 5, CD5, CD5 antigen (p56 62), CD5 antigen, CD5 Molecule, CD5_HUMAN, LEU 1, LEU1, Ly12, LyA, Lymphocyte Antigen CD5, Lymphocyte antigen T1/Leu 1, Lymphocyte antigen T1/Leu-1, Lymphocyte glycoprotein T1/Leu1, OTTHUMP00000236973, p56 62, T cell surface glycoprotein CD5, T-cell surface glycoprotein CD5, T1

    Background: CD5, also known as T1 or LEU1, is a cluster of differentiation found on a subset of IgM-secreting B cells called B-1 cells, and also on T cells. It is mapped to 11q12.2. Although CD5 can be found on both T cells and B cells, T cells express higher levels of CD5 than B cells. CD5 was used as a T-cell marker until monoclonal antibodies against CD3 were developed. CD5 is upregulated on T cells upon strong activation. In the thymus, there is a correlation with CD5 expression and strength of the interaction of the T cell towards self-peptides. Nevertheless, CD5 serves to mitigate activating signals from the BCR so that the B-1 cells can only be activated by very strong stimuli(such as bacterial proteins) and not by normal tissue proteins.

    Gene Name: CD5

    Production: Natural and recombinant human CD5. There is no detectable cross-reactivity with other relevant proteins.

    Standard: Expression system for standard: NSO, Immunogen sequence: R25-N371

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    Discover our top product CD5 ELISA Kit
  • 应用备注

    Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 6000pg/mL, 3000pg/mL, 1500pg/mL, 750pg/mL, 419pg/mL, 187.5pg/mL, 93.7pg/m human CD5 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human CD5 standard solution and each sample be measured in duplicate.

    Blood Product Anticoagulant: Heparin Sodium

    ELISA Dilution: 93.7pg/mL-6000pg/mL

    样本量
    100 μL
    板类型
    Pre-coated
    限制
    仅限研究用
  • 储存条件
    RT,4 °C,-20 °C
    储存方法
    Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
  • 抗原 See all CD5 ELISA试剂盒
    CD5
    别名
    soluble CD5 (CD5 产品)
    别名
    CD5 ELISA Kit, Ly-1 ELISA Kit, Ly-12 ELISA Kit, Ly-A ELISA Kit, Lyt-1 ELISA Kit, LEU1 ELISA Kit, T1 ELISA Kit, CD5 molecule ELISA Kit, CD5 antigen ELISA Kit, CD5 molecule like ELISA Kit, Cd5 molecule ELISA Kit, CD5 ELISA Kit, Cd5 ELISA Kit, CD5L ELISA Kit
    基因ID
    921
    NCBI登录号
    NP_055022
    UniProt
    P06127
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