S100A12 ELISA 试剂盒
Quick Overview for S100A12 ELISA 试剂盒 (ABIN6719725)
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检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 312 pg/mL
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Supplier Product No.
- koa0672
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Supplier
- Rockland
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原理
- Human S100A12 Sandwich ELISA Kit for Quantitative Detection
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品牌
- AccuSignal™
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Analytical Method
- Quantitative
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特异性
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Production: Natural and recombinant human S100A12. There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- < 10 pg/mL
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组件
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- Antibody-coated 96-well plate
- Target Protein Standard
- Detection antibody
- Detection reagent
- Diluent buffers
- Wash buffers
- Substrate Solution
- Stop solutions
- Adhesive covers
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S100 Calcium Binding Protein A12 (S100A12) ELISA Kit
S100A12 适用: 人 Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
S100 Calcium Binding Protein A12 (S100A12) ELISA KitS100A12 适用: 犬 Colorimetric Sandwich ELISA 0.625 ng/mL - 40 ng/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
S100 Calcium Binding Protein A12 (S100A12) ELISA KitS100A12 适用: 兔 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
S100 Calcium Binding Protein A12 (S100A12) ELISA KitS100A12 适用: 人 Colorimetric Sandwich ELISA 12.5-800 ng/mL Cell Culture Supernatant, Cerebrospinal Fluid, Serum
S100 Calcium Binding Protein A12 (S100A12) ELISA KitS100A12 适用: 人 AA 1-92 Colorimetric Sandwich ELISA 312-20.000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
S100 Calcium Binding Protein A12 (S100A12) ELISA KitS100A12 适用: Pig Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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应用备注
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ELISA_Dilution: 312pg/mL-20,000pg/mL
Application_Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 20,000pg/mL, 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL human S100A12 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma( heparin, EDTA) to each empty well. It is recommended that each human S100A12 standard solution and each sample be measured in duplicate.
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说明
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Standard: Expression system for standard: E.coli, Immunogen sequence: M1-E92
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样本量
- 100 μL
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板类型
- Pre-coated
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限制
- 仅限研究用
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
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有效期
- 12 months
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- S100A12 (S100 Calcium Binding Protein A12 (S100A12))
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别名
- S100A12
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背景
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Synonyms: CAAF1, CAGC, Calcitermin, Calcium binding protein in amniotic fluid, Calcium-binding protein in amniotic fluid 1, Calgranulin C, Calgranulin related protein, Calgranulin-C, CGRP, EN RAGE, EN-RAGE, ENRAGE, Extracellular newly identified RAGE-binding protein, migration inhibitory factor-related protein 6, MRP6, Neutrophil S100 protein, p6, Protein S100 A12, S100 calcium binding protein A12, S100 calcium-binding protein A12 (calgranulin C), S100 calcium-binding protein A12, S10AC_HUMAN
Background: S100 calcium-binding protein A12 (S100A12) is a protein that in humans is encoded by the S100A12 gene. It is also known as calgranulin C. By direct R-banding FISH, the S100A12 gene is mapped to chromosome 1q21.2-q22. The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. And this protein is proposed to be involved in specific calcium-dependent signal transduction pathways and its regulatory effect on cytoskeletal components may modulate various neutrophil activities.
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基因ID
- 6283
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NCBI登录号
- NP_005612
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UniProt
- P80511
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途径
- Toll-Like Receptors Cascades, S100 Proteins
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