CEACAM1 ELISA 试剂盒
Our Local Distributor
北京 101111
Quick Overview for CEACAM1 ELISA 试剂盒 (ABIN6719724)
抗原
See all CEACAM1 ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
-
-
最低检测浓度
- 312 pg/mL
-
Supplier Product No.
- koa0822
-
Supplier
- Rockland
-
原理
- Human CEACAM1 Sandwich ELISA Kit for Quantitative Detection
-
品牌
- AccuSignal™
-
Analytical Method
- Quantitative
-
特异性
-
Expression system for standard: NS0
Immunogen sequence: Q35-G428 -
交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
-
灵敏度
- < 10 pg/mL
-
组件
-
- Antibody-coated 96-well plate
- Target Protein Standard
- Detection antibody
- Detection reagent
- Diluent buffers
- Wash buffers
- Substrate Solution
- Stop solutions
- Adhesive covers
-
试剂未包括
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.
-
-
Carcinoembryonic Antigen-Related Cell Adhesion Molecule 1 (CEACAM1) ELISA Kit
CEACAM1 适用: 人 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Plasma, Serum
Carcinoembryonic Antigen-Related Cell Adhesion Molecule 1 (CEACAM1) ELISA KitCEACAM1 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Carcinoembryonic Antigen-Related Cell Adhesion Molecule 1 (CEACAM1) ELISA KitCEACAM1 适用: 小鼠 Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
Carcinoembryonic Antigen-Related Cell Adhesion Molecule 1 (CEACAM1) ELISA KitCEACAM1 适用: 人 AA 35-428 Colorimetric Sandwich ELISA 312 pg/mL - 20000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma (EDTA), Plasma (heparin), Serum
Carcinoembryonic Antigen-Related Cell Adhesion Molecule 1 (CEACAM1) ELISA KitCEACAM1 适用: 人 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Plasma, Serum
Carcinoembryonic Antigen-Related Cell Adhesion Molecule 1 (CEACAM1) ELISA KitCEACAM1 适用: 人 Colorimetric Sandwich ELISA 468.75 pg/mL - 30000 pg/mL Cell Culture Supernatant, Plasma, Serum
Carcinoembryonic Antigen-Related Cell Adhesion Molecule 1 (CEACAM1) ELISA KitCEACAM1 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
-
-
说明
-
Capture antibody and Detection antibody: monoclonal antibody from mouse, polyclonal antibody from goat
-
样本量
- 100 μL
-
板类型
- Pre-coated
-
实验流程
- Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 20,000pg/mL, 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL human CEACAM1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human CEACAM1 standard solution and each sample be measured in duplicate.
-
实验精密度
-
Intra-Assay Precision (CV%): 7%, 5.3%, 4.8%
Inter-Assay Precision (CV%): 8.2%, 6.9%, 5.4% -
限制
- 仅限研究用
-
-
-
储存条件
- 4 °C,-20 °C
-
储存方法
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
-
有效期
- 12 months
-
-
- CEACAM1 (Carcinoembryonic Antigen-Related Cell Adhesion Molecule 1 (CEACAM1))
-
别名
- CEACAM1
-
背景
-
Background: CEACAM1, also known as CD66a (Cluster of Differentiation 66a), is a human glycoprotein encoded by a gene. This gene encodes a member of the carcinoembryonic antigen (CEA) gene family, which belongs to the immunoglobulin superfamily. CEACAM1 is mapped to 19q13.2 in human. The encoded protein was originally described in bile ducts of liver as biliary glycoprotein. Subsequently, it was found to be a cell-cell adhesion molecule detected on leukocytes, epithelia, and endothelia. The encoded protein mediates cell adhesion via homophilic as well as heterophilic binding to other proteins of the subgroup. Multiple cellular activities have been attributed to the encoded protein, including roles in the differentiation and arrangement of tissue three-dimensional structure, angiogenesis, apoptosis, tumor suppression, metastasis, and the modulation of innate and adaptive immune responses.
Gene Full Name: CEA cell adhesion molecule 1
-
基因ID
- 634
-
NCBI登录号
- NP_001020083
-
UniProt
- P13688
抗原 See all CEACAM1 ELISA试剂盒
-
