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IL7R ELISA 试剂盒

This 小鼠 IL7R ELISA Kit is a Colorimetric ELISA Kit designed to quantify 小鼠 IL7R.
Rockland
产品编号 ABIN6719685
Supplier Product No.: koa0416
发货至: 中国

Quick Overview for IL7R ELISA 试剂盒 (ABIN6719685)

抗原

See all IL7R ELISA试剂盒
IL7R (Interleukin 7 Receptor (IL7R))

适用

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小鼠

检测方法

Colorimetric

实验类型

Sandwich ELISA

检测范围

312 pg/mL - 20000 pg/mL

应用范围

ELISA

样品类型

Cell Culture Supernatant, Serum
  • 最低检测浓度

    312 pg/mL

    Supplier Product No.

    koa0416

    Supplier

    Rockland

    原理

    Mouse IL-7R - CD127 Sandwich ELISA Kit for Quantitative Detection

    品牌

    AccuSignal™

    Analytical Method

    Quantitative

    特异性

    Production: Natural and recombinant mouse IL7R. There is no detectable cross-reactivity with other relevant proteins.

    灵敏度

    < 10 pg/mL

    组件

    • Antibody-coated 96-well plate
    • Target Protein Standard
    • Detection antibody
    • Detection reagent
    • Diluent buffers
    • Wash buffers
    • Substrate Solution
    • Stop solutions
    • Adhesive covers
  • 应用备注

    ELISA_Dilution: 312 pg/mL-20000 pg/mL

    Application_Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 20000pg/mL, 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL mouse IL7R standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates or serum to each empty well. It is recommended that each mouse IL7R standard solution and each sample be measured in duplicate.

    说明

    Standard: Expression system for standard: NSO, Immunogen sequence: E21-D239

    样本量

    100 μL

    板类型

    Pre-coated

    限制

    仅限研究用
  • 储存条件

    4 °C,-20 °C

    储存方法

    Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

    有效期

    12 months
  • 抗原 See all IL7R ELISA试剂盒

    IL7R (Interleukin 7 Receptor (IL7R))

    别名

    IL7R/CD127

    背景

    Synonyms: CD 127, CD127, CD127 antigen, CDw127, IL 7R alpha, IL 7R, IL-7 receptor subunit alpha, IL-7R subunit alpha, IL-7R-alpha, IL-7RA, IL7R, IL7RA, IL7RA_HUMAN, IL7Ralpha, ILRA, Interleukin 7 receptor alpha chain, Interleukin 7 receptor, Interleukin 7 receptor isoform H5 6, Interleukin-7 receptor subunit alpha

    Background: The interleukin-7 receptor(IL7R) is a protein found on the surface of cells. It is mapped to 5p13.2. The protein encoded by this gene is a receptor for interleukine 7(IL7). The function of this receptor requires the interleukin 2 receptor, gamma chain(IL2RG), which is a common gamma chain shared by the receptors of various cytokines, including interleukine 2, 4, 7, 9, and 15. IL7R has been shown to play a critical role in the development of immune cells called lymphocytes-specifically in a process known as V(D)J recombination. This protein is also found to control the accessibility of a region of the genome that contains the T-cell receptor gamma gene, by STAT5 and histone acetylation. What's more, IL7R antagonism is efficacious in treatment of EAE through its effects on Th17 cells and is a potential treatment for MS.

    基因ID

    16197

    NCBI登录号

    NP_032398

    UniProt

    P16872

    途径

    JAK/STAT Signaling, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Regulation of Cell Size
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