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Chordin ELISA 试剂盒

CHRD 适用: 小鼠 Colorimetric Sandwich ELISA 49-12.000 pg/mL Cell Culture Supernatant, Plasma, Serum
产品编号 ABIN625379
发货至: 中国
  • 抗原 See all Chordin (CHRD) ELISA试剂盒
    Chordin (CHRD)
    适用
    • 4
    • 1
    • 1
    小鼠
    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    检测范围
    49-12.000 pg/mL
    最低检测浓度
    49 pg/mL
    应用范围
    ELISA
    原理
    Mouse Chordin ELISA Kit for Cell Culture Supernatants, EDTA and/or Citrate treated plasma, and Serum samples. Heparin is not recommended.
    样品类型
    Cell Culture Supernatant, Plasma, Serum
    Analytical Method
    Quantitative
    特异性
    The antibody pair provided in this kit recognizes mouse Chordin.
    灵敏度
    49 pg/mL
    产品特性
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    组件
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    试剂未包括
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
    Top Product
    Discover our top product CHRD ELISA Kit
  • 应用备注
    Recommended Dilution for serum and plasma samples2 fold
    样本量
    100 μL
    板类型
    Pre-coated
    实验流程
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    样品制备

    Collect plasma using EDTA and/or Citrate as the anticoagulant. Heparin is not recommended.

    限制
    仅限研究用
  • 储存条件
    -20 °C
    储存方法
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    有效期
    6 months
  • 抗原 See all Chordin (CHRD) ELISA试剂盒
    Chordin (CHRD)
    别名
    Chordin (CHRD 产品)
    别名
    chd ELISA Kit, X-chordin ELISA Kit, Chd ELISA Kit, chordino ELISA Kit, wu:fe47d04 ELISA Kit, chordin ELISA Kit, chordin, gene 1 ELISA Kit, chordin, gene 1 S homeolog ELISA Kit, CHRD ELISA Kit, chrd.1 ELISA Kit, LOC778563 ELISA Kit, Chrd ELISA Kit, chrd ELISA Kit, chrd.1.S ELISA Kit
    背景
    Gene Names: Chrd
    Protein names: Chordin
    基因ID
    12667
    UniProt
    Q9Z0E2
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