CD56 ELISA 试剂盒
(2 references)-
- 抗原 See all CD56 (NCAM1) ELISA试剂盒
- CD56 (NCAM1) (Neural Cell Adhesion Molecule 1 (NCAM1))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.5 pg/mL-150 ng/mL
- 最低检测浓度
- 0.5 pg/mL
- 应用范围
- ELISA
- 原理
- Human NCAM-1 (CD56) ELISA Kit for cell culture supernatants, plasma, and serum samples.
- 样品类型
- Cell Culture Supernatant, Plasma, Serum
- Analytical Method
- Quantitative
- 特异性
- Cross Reactivity: This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF, BLC, CNTF, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-4, IL-5, IL-6, IL-8, IL-9, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, FGF-4, FGF-6, FGF-7, G-CSF, GDNF, GM-CSF, IFN-gamma, IGFBP-2, IGFBP-3, IGFBP-4, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIF, MIG, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF,SDF-1 alpha, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
- 灵敏度
- 0.5 pg/mL
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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- Discover our top product NCAM1 ELISA Kit
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Neural Cell Adhesion Molecule 1 (NCAM1) ELISA Kit
NCAM1 适用: 人 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Culture Supernatant, Plasma, Serum
Neural Cell Adhesion Molecule 1 (NCAM1) ELISA KitNCAM1 适用: 人 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
Neural Cell Adhesion Molecule 1 (NCAM1) ELISA KitNCAM1 适用: 人 AA 20-718 Colorimetric Sandwich ELISA 156-10000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Neural Cell Adhesion Molecule 1 (NCAM1) ELISA KitNCAM1 适用: 猴 AA 20-718 Colorimetric Sandwich ELISA
Neural Cell Adhesion Molecule 1 (NCAM1) ELISA KitNCAM1 适用: 小鼠 AA 20-711 Colorimetric Sandwich ELISA 625-40.000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Neural Cell Adhesion Molecule 1 (NCAM1) ELISA KitNCAM1 适用: 大鼠 AA 20-712 Colorimetric Sandwich ELISA
Neural Cell Adhesion Molecule 1 (NCAM1) ELISA KitNCAM1 适用: 人 Colorimetric Sandwich ELISA 156 pg/mL - 10000 pg/mL Cell Culture Lysate, Cell Culture Supernatant, Plasma (EDTA - heparin), Serum, Tissue Homogenate
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- 应用备注
- Recommended Dilution for serum and plasma samples10-100 fold
- 样本量
- 100 μL
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 储存条件
- -20 °C
- 储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- 有效期
- 6 months
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: "Low neural exosomal levels of cellular survival factors in Alzheimer's disease." in: Annals of clinical and translational neurology, Vol. 2, Issue 7, pp. 769-73, (2015) (PubMed).
: "Promoter variant rs2301228 on the neural cell adhesion molecule 1 gene confers risk of schizophrenia in Han Chinese." in: Schizophrenia research, Vol. 160, Issue 1-3, pp. 88-96, (2014) (PubMed).
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: "Low neural exosomal levels of cellular survival factors in Alzheimer's disease." in: Annals of clinical and translational neurology, Vol. 2, Issue 7, pp. 769-73, (2015) (PubMed).
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- 抗原 See all CD56 (NCAM1) ELISA试剂盒
- CD56 (NCAM1) (Neural Cell Adhesion Molecule 1 (NCAM1))
- 别名
- N-CAM1 (NCAM1 产品)
- 别名
- CD56 ELISA Kit, MSK39 ELISA Kit, NCAM ELISA Kit, fj56g09 ELISA Kit, ncam ELISA Kit, ncam1 ELISA Kit, wu:fj56g09 ELISA Kit, NCAM1 ELISA Kit, n-cam ELISA Kit, E-NCAM ELISA Kit, NCAM-1 ELISA Kit, Ncam ELISA Kit, Cd56 ELISA Kit, N-CAM ELISA Kit, N-CAM-1 ELISA Kit, NCAM-C ELISA Kit, NCAMC ELISA Kit, N-CAM-1-B ELISA Kit, NCAM-1-B ELISA Kit, ncam1-a ELISA Kit, ncam1-b ELISA Kit, neural cell adhesion molecule 1 ELISA Kit, neural cell adhesion molecule 1a ELISA Kit, neural cell adhesion molecule 1 S homeolog ELISA Kit, neural cell adhesion molecule 1 L homeolog ELISA Kit, NCAM1 ELISA Kit, ncam1a ELISA Kit, ncam1 ELISA Kit, LOC100085373 ELISA Kit, ncam1.S ELISA Kit, Ncam1 ELISA Kit, ncam1.L ELISA Kit
- 背景
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Gene Names: NCAM1 NCAM
Protein names: Neural cell adhesion molecule 1 (N-CAM-1) (NCAM-1) (CD antigen CD56)
- 基因ID
- 4684
- UniProt
- P13591
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