IL10RB ELISA 试剂盒
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- 抗原 See all IL10RB ELISA试剂盒
- IL10RB (Interleukin 10 Receptor, beta (IL10RB))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 5-3000 pg/mL
- 最低检测浓度
- 5 pg/mL
- 应用范围
- ELISA
- 原理
- Human IL-10 R beta ELISA Kit for cell culture supernatants, plasma, and serum samples.
- 样品类型
- Plasma, Cell Culture Supernatant, Serum
- Analytical Method
- Quantitative
- 特异性
- This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF, BLC, ENA-78, FGF- 4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
- 灵敏度
- 5 pg/mL
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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- Discover our best selling IL10RB ELISA Kit
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- Discover our top product IL10RB ELISA Kit
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Interleukin 10 Receptor, beta (IL10RB) ELISA Kit
IL10RB 适用: 人 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Interleukin 10 Receptor, beta (IL10RB) ELISA KitIL10RB 适用: 人 Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
Interleukin 10 Receptor, beta (IL10RB) ELISA KitIL10RB 适用: 大鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
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- 应用备注
- Recommended Dilution for serum and plasma samples2 fold
- 样本量
- 100 μL
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 储存条件
- -20 °C
- 储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- 有效期
- 6 months
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- 抗原 See all IL10RB ELISA试剂盒
- IL10RB (Interleukin 10 Receptor, beta (IL10RB))
- 别名
- IL-10 R beta (IL10RB 产品)
- 别名
- IL10R2 ELISA Kit, IL10RB ELISA Kit, il10r2 ELISA Kit, CDW210B ELISA Kit, CRF2-4 ELISA Kit, CRFB4 ELISA Kit, D21S58 ELISA Kit, D21S66 ELISA Kit, IL-10R2 ELISA Kit, 6620401D04Rik ELISA Kit, AI528744 ELISA Kit, Crfb4 ELISA Kit, D16H21S58 ELISA Kit, D21S58h ELISA Kit, Il10r2 ELISA Kit, RGD1560373 ELISA Kit, interleukin 10 receptor subunit beta ELISA Kit, interleukin 10 receptor subunit beta S homeolog ELISA Kit, interleukin-10 receptor subunit beta ELISA Kit, interleukin 10 receptor, beta ELISA Kit, IL10RB ELISA Kit, il10rb.S ELISA Kit, LOC473966 ELISA Kit, LOC478404 ELISA Kit, il10rb ELISA Kit, Il10rb ELISA Kit
- 背景
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Gene Names: IL10RB CRFB4 D21S58 D21S66
Protein names: Interleukin-10 receptor subunit beta (IL-10 receptor subunit beta) (IL-10R subunit beta) (IL-10RB) (Cytokine receptor class-II member 4) (Cytokine receptor family 2 member 4) (CRF2-4) (Interleukin-10 receptor subunit 2) (IL-10R subunit 2) (IL-10R2) (CD antigen CDw210b)
- 基因ID
- 3588
- UniProt
- Q08334
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