ICAM2 ELISA 试剂盒
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Quick Overview for ICAM2 ELISA 试剂盒 (ABIN625306)
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检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 0.17 ng/mL
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原理
- Human ICAM-2/CD102 ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.
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Analytical Method
- Quantitative
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特异性
- This ELISA kit shows no cross-reactivity with the following cytokines tested: human Activin A, AgRP, Angiogenin, Angiopoietin-1, Angiostatin, Cathepsin S, CD40 (TNFRSF5), Cripto-1, DAN, DKK-1, ECadherin, Fas Ligand (TNFSF6), Fc gamma RIIB/C (CD32B/C), Follistatin, Galectin-7, gp130, IL-1 R4 (ST2), IL-13 R alpha 2, IL-13 R1, IL-17B, IL-2 R alpha, IL-2 R beta (CD122), IL-23, LAP/TGF beta 1, NrCAM, PAI-1, PDGF-AB, Resistin, SDF-1 beta (CXCL12 beta), Siglec-5 (CD170), Sonic Hedgehog N-Terminal (Shh-N), TGF beta 2, Thrombopoietin (TPO), Tie-2, TRAIL R4 (TNFRSF10D), TREM-1, TROP1 (EpCAM), VEGF-C, VEGFR1.
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产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
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组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
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试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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Intercellular Adhesion Molecule 2 (ICAM2) ELISA Kit
ICAM2 适用: 小鼠 Colorimetric Sandwich ELISA 0.391 ng/mL - 25 ng/mL Plasma, Serum, Tissue Homogenate
Intercellular Adhesion Molecule 2 (ICAM2) ELISA KitICAM2 适用: 人 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Intercellular Adhesion Molecule 2 (ICAM2) ELISA KitICAM2 适用: 大鼠 Colorimetric Sandwich ELISA 0.781 ng/mL - 50 ng/mL Plasma, Serum, Tissue Homogenate
Intercellular Adhesion Molecule 2 (ICAM2) ELISA KitICAM2 适用: 小鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum
Intercellular Adhesion Molecule 2 (ICAM2) ELISA KitICAM2 适用: 人 AA 25-223 Colorimetric Sandwich ELISA 312 pg/mL - 20000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Intercellular Adhesion Molecule 2 (ICAM2) ELISA KitICAM2 适用: 小鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum
Intercellular Adhesion Molecule 2 (ICAM2) ELISA KitICAM2 适用: 人 Colorimetric Sandwich ELISA 312 pg/mL - 20000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Intercellular Adhesion Molecule 2 (ICAM2) ELISA KitICAM2 适用: 大鼠 Colorimetric Sandwich ELISA 0.781 ng/mL - 50 ng/mL Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
Intercellular Adhesion Molecule 2 (ICAM2) ELISA KitICAM2 适用: 小鼠 Colorimetric Sandwich ELISA 0.391 ng/mL - 25 ng/mL Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
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应用备注
- Suggested Plasma Dilution: 50 fold
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板类型
- Pre-coated
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实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
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限制
- 仅限研究用
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储存条件
- -20 °C
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储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
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有效期
- 6 months
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- ICAM2 (Intercellular Adhesion Molecule 2 (ICAM2))
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别名
- ICAM-2
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背景
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Gene Symbols: ICAM2
Protein Name (Synonyms): Intercellular adhesion molecule 2 (ICAM-2) (CD antigen CD102)
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基因ID
- 3384
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UniProt
- P13598
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