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TIMP2 ELISA 试剂盒

Name: TIMP2 ELISA 试剂盒
SKU: ABIN625100
Availability: OutOfStock

This 人 TIMP2 ELISA Kit is a Colorimetric ELISA Kit designed to quantify 人 TIMP2. This ELISA Kit has been cited in 20+ publications.

ELISA

1 image

(20 references)

产品编号 ABIN625100

发货至: 中国

Quick Overview for TIMP2 ELISA 试剂盒 (ABIN625100)

抗原

See all TIMP2 ELISA试剂盒

TIMP2 (Metalloproteinase Inhibitor 2 (TIMP2))

适用

人

检测方法

Colorimetric

实验类型

Sandwich ELISA

检测范围

25-7000 pg/mL

应用范围

ELISA

样品类型

Plasma, Cell Culture Supernatant, Serum

Discover our top product TIMP2 ELISA Kit

最低检测浓度

25 pg/mL

原理

Human TIMP-2 ELISA Kit for cell culture supernatants, plasma, and serum samples.

Analytical Method

Quantitative

特异性

This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, G-CSF, GM-CSF, IFN-gamma, MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, PDGF, SCF, TNF-alpha, TNF-beta, TPO, VEGF.

灵敏度

2 pg/mL

产品特性
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
组件
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
试剂未包括
- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
Metalloproteinase Inhibitor 2 (TIMP2) ELISA Kit
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Metalloproteinase Inhibitor 2 (TIMP2) ELISA Kit
TIMP2 适用: 大鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Metalloproteinase Inhibitor 2 (TIMP2) ELISA Kit
TIMP2 适用: 人 Colorimetric Sandwich ELISA 0.94 ng/mL - 60 ng/mL Plasma, Serum, Tissue Homogenate

Metalloproteinase Inhibitor 2 (TIMP2) ELISA Kit
TIMP2 适用: Pig Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate

Metalloproteinase Inhibitor 2 (TIMP2) ELISA Kit
TIMP2 适用: 马 Colorimetric Sandwich ELISA 7.8 ng/mL - 500 ng/mL Plasma, Serum, Tissue Homogenate

Metalloproteinase Inhibitor 2 (TIMP2) ELISA Kit
TIMP2 适用: 人 Colorimetric Sandwich ELISA 0.16 ng/mL - 10 ng/mL Cell Culture Supernatant, Plasma, Serum

Metalloproteinase Inhibitor 2 (TIMP2) ELISA Kit
Wide Range TIMP2 适用: 大鼠 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Metalloproteinase Inhibitor 2 (TIMP2) ELISA Kit
TIMP2 适用: 小鸡 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate

Metalloproteinase Inhibitor 2 (TIMP2) ELISA Kit
TIMP2 适用: 豚鼠 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate

Metalloproteinase Inhibitor 2 (TIMP2) ELISA Kit
TIMP2 适用: 兔 Colorimetric Sandwich ELISA 0.781 ng/mL - 50 ng/mL Plasma, Serum, Tissue Homogenate

应用备注

Recommended Dilution for serum and plasma samples300 - 3,000 fold

样本量

100 μL

板类型

Pre-coated

实验流程
1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.
限制

仅限研究用
储存条件

-20 °C

储存方法

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

有效期

6 months
Prato, Khadjavi, Magnetto, Gulino, Rolfo, Todros, Cavalli, Guiot: "Effects of oxygen tension and dextran-shelled/2H,3H-decafluoropentane-cored oxygen-loaded nanodroplets on secretion of gelatinases and their inhibitors in term human placenta." in: Bioscience, biotechnology, and biochemistry, Vol. 80, Issue 3, pp. 466-72, (2016) (PubMed).

Gulino, Magnetto, Khadjavi, Panariti, Rivolta, Soster, Argenziano, Cavalli, Giribaldi, Guiot, Prato: "Oxygen-Loaded Nanodroplets Effectively Abrogate Hypoxia Dysregulating Effects on Secretion of MMP-9 and TIMP-1 by Human Monocytes." in: Mediators of inflammation, Vol. 2015, pp. 964838, (2015) (PubMed).

Garratt, Sutanto, Ling, Looi, Iosifidis, Martinovich, Shaw, Kicic-Starcevich, Knight, Ranganathan, Stick, Kicic: "Matrix metalloproteinase activation by free neutrophil elastase contributes to bronchiectasis progression in early cystic fibrosis." in: The European respiratory journal, Vol. 46, Issue 2, pp. 384-94, (2015) (PubMed).

Khadjavi, Magnetto, Panariti, Argenziano, Gulino, Rivolta, Cavalli, Giribaldi, Guiot, Prato: "Chitosan-shelled oxygen-loaded nanodroplets abrogate hypoxia dysregulation of human keratinocyte gelatinases and inhibitors: New insights for chronic wound healing." in: Toxicology and applied pharmacology, Vol. 286, Issue 3, pp. 198-206, (2015) (PubMed).

Ciaffi, Cavassini, Genne, Delhumeau, Spycher Elbes, Hill, Wandeler, Fehr, Stoeckle, Schmid, Hirschel, Montecucco, Calmy: "Switch to etravirine for HIV-positive patients receiving statin treatment: a prospective study." in: European journal of clinical investigation, Vol. 45, Issue 7, pp. 720-30, (2015) (PubMed).

Basilico, Magnetto, DAlessandro, Panariti, Rivolta, Genova, Khadjavi, Gulino, Argenziano, Soster, Cavalli, Giribaldi, Guiot, Prato: "Dextran-shelled oxygen-loaded nanodroplets reestablish a normoxia-like pro-angiogenic phenotype and behavior in hypoxic human dermal microvascular endothelium." in: Toxicology and applied pharmacology, Vol. 288, Issue 3, pp. 330-8, (2015) (PubMed).

Koob, Lim, Zabek, Massee: "Cytokines in single layer amnion allografts compared to multilayer amnion/chorion allografts for wound healing." in: Journal of biomedical materials research. Part B, Applied biomaterials, Vol. 103, Issue 5, pp. 1133-40, (2015) (PubMed).

Kiliç, Uçar, Özdemir, Colak, Bal, Ertu?rul: "Circulating matrix metalloproteinases and tissue inhibitors of metalloproteinases levels in pediatric patients with congenital heart disease: Relationship to cardiac functions." in: Anadolu kardiyoloji dergisi : AKD = the Anatolian journal of cardiology, Vol. 14, Issue 6, pp. 531-41, (2014) (PubMed).

Richards, Timulak, Doherty, Sharry, Colla, Joyce, Hayes: "Internet-delivered treatment: its potential as a low-intensity community intervention for adults with symptoms of depression: protocol for a randomized controlled trial." in: BMC psychiatry, Vol. 14, pp. 147, (2014) (PubMed).

Hampel, Klonisch, Sel, Schulze, Garreis, Seitmann, Zouboulis, Paulsen: "Insulin-like factor 3 promotes wound healing at the ocular surface." in: Endocrinology, Vol. 154, Issue 6, pp. 2034-45, (2013) (PubMed).

Chon, Kim, Ahn: "Transient elastography in real practice: a time for action?" in: Journal of gastroenterology and hepatology, Vol. 27, Issue 7, pp. 1134-5, (2012) (PubMed).

Kaminska-Winciorek, Deja, Polanska, Jarosz-Chobot: "The role of selected metalloproteinases in cheiroarthropathy in children with type 1 diabetes - a pilotage study." in: International journal of clinical practice, Vol. 66, Issue 4, pp. 374-7, (2012) (PubMed).

Müller, Kainov, El Bakkouri, Saelens, De Brabander, Kittel, Samm, Muller: "The proton translocation domain of cellular vacuolar ATPase provides a target for the treatment of influenza A virus infections." in: British journal of pharmacology, Vol. 164, Issue 2, pp. 344-57, (2012) (PubMed).

Mamlouk, Balagurumoorthy, Wang, Adelstein, Kassis: "Bystander effect in tumor cells produced by Iodine-125 labeled human lymphocytes." in: International journal of radiation biology, Vol. 88, Issue 12, pp. 1019-27, (2012) (PubMed).

Moir, Ng, Poniris, Santa, Burgess, Oliver, Krymskaya, Black: "Doxycycline inhibits matrix metalloproteinase-2 secretion from TSC2-null mouse embryonic fibroblasts and lymphangioleiomyomatosis cells." in: British journal of pharmacology, Vol. 164, Issue 1, pp. 83-92, (2012) (PubMed).

Steed, Trumpower, Duffy, Smith, Marshall, Rupp, Robson: "Amnion-derived cellular cytokine solution: a physiological combination of cytokines for wound healing." in: Eplasty, Vol. 8, pp. e18, (2008) (PubMed).

Malard, Berenguer, Prat, Ruat, Steinmetz, Quemeneur: "Global gene expression profiling in human lung cells exposed to cobalt." in: BMC genomics, Vol. 8, pp. 147, (2007) (PubMed).

Burns, Griffith, Waldhausen: "A linear oscillating electromotor for possible application in an intrathoracic artificial heart." in: Transactions - American Society for Artificial Internal Organs, Vol. 10, pp. 151-3 (PubMed).

Hickman, Sargent: "The application of the use of radioactive phosphorus (32P) in larvae: a new approach for identification of adult insect sources." in: Investigative radiology, Vol. 1, Issue 2, pp. 104-5 (PubMed).

Jay: "Production of lysozyme by staphylococci and its correlation with three other extracellular substances." in: Journal of bacteriology, Vol. 91, Issue 5, pp. 1804-10 (PubMed).

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抗原 See all TIMP2 ELISA试剂盒

TIMP2 (Metalloproteinase Inhibitor 2 (TIMP2))

别名

TIMP-2

背景

Gene Names: TIMP2
Protein names: Metalloproteinase inhibitor 2 (CSC-21K) (Tissue inhibitor of metalloproteinases 2) (TIMP-2)

基因ID

7077

UniProt

P16035

途径

cAMP Metabolic Process