TIMP2 ELISA 试剂盒
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Quick Overview for TIMP2 ELISA 试剂盒 (ABIN625100)
抗原
See all TIMP2 ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 25 pg/mL
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原理
- Human TIMP-2 ELISA Kit for cell culture supernatants, plasma, and serum samples.
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Analytical Method
- Quantitative
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特异性
- This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, G-CSF, GM-CSF, IFN-gamma, MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, PDGF, SCF, TNF-alpha, TNF-beta, TPO, VEGF.
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灵敏度
- 2 pg/mL
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产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
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组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
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试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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Metalloproteinase Inhibitor 2 (TIMP2) ELISA Kit
TIMP2 适用: 小鼠 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Metalloproteinase Inhibitor 2 (TIMP2) ELISA KitTIMP2 适用: 大鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Metalloproteinase Inhibitor 2 (TIMP2) ELISA KitTIMP2 适用: 人 Colorimetric Sandwich ELISA 0.94 ng/mL - 60 ng/mL Plasma, Serum, Tissue Homogenate
Metalloproteinase Inhibitor 2 (TIMP2) ELISA KitTIMP2 适用: Pig Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Metalloproteinase Inhibitor 2 (TIMP2) ELISA KitTIMP2 适用: 马 Colorimetric Sandwich ELISA 7.8 ng/mL - 500 ng/mL Plasma, Serum, Tissue Homogenate
Metalloproteinase Inhibitor 2 (TIMP2) ELISA KitWide Range TIMP2 适用: 大鼠 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Metalloproteinase Inhibitor 2 (TIMP2) ELISA KitTIMP2 适用: 小鸡 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Metalloproteinase Inhibitor 2 (TIMP2) ELISA KitTIMP2 适用: 豚鼠 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Metalloproteinase Inhibitor 2 (TIMP2) ELISA KitTIMP2 适用: 兔 Colorimetric Sandwich ELISA 0.781 ng/mL - 50 ng/mL Plasma, Serum, Tissue Homogenate
Metalloproteinase Inhibitor 2 (TIMP2) ELISA KitSmall Sample TIMP2 适用: 小鼠 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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应用备注
- Recommended Dilution for serum and plasma samples300 - 3,000 fold
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样本量
- 100 μL
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板类型
- Pre-coated
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实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
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限制
- 仅限研究用
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储存条件
- -20 °C
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储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
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有效期
- 6 months
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: "Effects of oxygen tension and dextran-shelled/2H,3H-decafluoropentane-cored oxygen-loaded nanodroplets on secretion of gelatinases and their inhibitors in term human placenta." in: Bioscience, biotechnology, and biochemistry, Vol. 80, Issue 3, pp. 466-72, (2016) (PubMed).
: "Oxygen-Loaded Nanodroplets Effectively Abrogate Hypoxia Dysregulating Effects on Secretion of MMP-9 and TIMP-1 by Human Monocytes." in: Mediators of inflammation, Vol. 2015, pp. 964838, (2015) (PubMed).
: "Matrix metalloproteinase activation by free neutrophil elastase contributes to bronchiectasis progression in early cystic fibrosis." in: The European respiratory journal, Vol. 46, Issue 2, pp. 384-94, (2015) (PubMed).
: "Chitosan-shelled oxygen-loaded nanodroplets abrogate hypoxia dysregulation of human keratinocyte gelatinases and inhibitors: New insights for chronic wound healing." in: Toxicology and applied pharmacology, Vol. 286, Issue 3, pp. 198-206, (2015) (PubMed).
: "Switch to etravirine for HIV-positive patients receiving statin treatment: a prospective study." in: European journal of clinical investigation, Vol. 45, Issue 7, pp. 720-30, (2015) (PubMed).
: "Dextran-shelled oxygen-loaded nanodroplets reestablish a normoxia-like pro-angiogenic phenotype and behavior in hypoxic human dermal microvascular endothelium." in: Toxicology and applied pharmacology, Vol. 288, Issue 3, pp. 330-8, (2015) (PubMed).
: "Cytokines in single layer amnion allografts compared to multilayer amnion/chorion allografts for wound healing." in: Journal of biomedical materials research. Part B, Applied biomaterials, Vol. 103, Issue 5, pp. 1133-40, (2015) (PubMed).
: "Circulating matrix metalloproteinases and tissue inhibitors of metalloproteinases levels in pediatric patients with congenital heart disease: Relationship to cardiac functions." in: Anadolu kardiyoloji dergisi : AKD = the Anatolian journal of cardiology, Vol. 14, Issue 6, pp. 531-41, (2014) (PubMed).
: "Internet-delivered treatment: its potential as a low-intensity community intervention for adults with symptoms of depression: protocol for a randomized controlled trial." in: BMC psychiatry, Vol. 14, pp. 147, (2014) (PubMed).
: "Insulin-like factor 3 promotes wound healing at the ocular surface." in: Endocrinology, Vol. 154, Issue 6, pp. 2034-45, (2013) (PubMed).
: "Transient elastography in real practice: a time for action?" in: Journal of gastroenterology and hepatology, Vol. 27, Issue 7, pp. 1134-5, (2012) (PubMed).
: "The role of selected metalloproteinases in cheiroarthropathy in children with type 1 diabetes - a pilotage study." in: International journal of clinical practice, Vol. 66, Issue 4, pp. 374-7, (2012) (PubMed).
: "The proton translocation domain of cellular vacuolar ATPase provides a target for the treatment of influenza A virus infections." in: British journal of pharmacology, Vol. 164, Issue 2, pp. 344-57, (2012) (PubMed).
: "Bystander effect in tumor cells produced by Iodine-125 labeled human lymphocytes." in: International journal of radiation biology, Vol. 88, Issue 12, pp. 1019-27, (2012) (PubMed).
: "Doxycycline inhibits matrix metalloproteinase-2 secretion from TSC2-null mouse embryonic fibroblasts and lymphangioleiomyomatosis cells." in: British journal of pharmacology, Vol. 164, Issue 1, pp. 83-92, (2012) (PubMed).
: "Amnion-derived cellular cytokine solution: a physiological combination of cytokines for wound healing." in: Eplasty, Vol. 8, pp. e18, (2008) (PubMed).
: "Global gene expression profiling in human lung cells exposed to cobalt." in: BMC genomics, Vol. 8, pp. 147, (2007) (PubMed).
: "A linear oscillating electromotor for possible application in an intrathoracic artificial heart." in: Transactions - American Society for Artificial Internal Organs, Vol. 10, pp. 151-3 (PubMed).
: "The application of the use of radioactive phosphorus (32P) in larvae: a new approach for identification of adult insect sources." in: Investigative radiology, Vol. 1, Issue 2, pp. 104-5 (PubMed).
: "Production of lysozyme by staphylococci and its correlation with three other extracellular substances." in: Journal of bacteriology, Vol. 91, Issue 5, pp. 1804-10 (PubMed).
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: "Effects of oxygen tension and dextran-shelled/2H,3H-decafluoropentane-cored oxygen-loaded nanodroplets on secretion of gelatinases and their inhibitors in term human placenta." in: Bioscience, biotechnology, and biochemistry, Vol. 80, Issue 3, pp. 466-72, (2016) (PubMed).
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- TIMP2 (Metalloproteinase Inhibitor 2 (TIMP2))
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别名
- TIMP-2
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背景
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Gene Names: TIMP2
Protein names: Metalloproteinase inhibitor 2 (CSC-21K) (Tissue inhibitor of metalloproteinases 2) (TIMP-2)
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基因ID
- 7077
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UniProt
- P16035
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途径
- cAMP Metabolic Process
抗原 See all TIMP2 ELISA试剂盒
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